A finite-cluster phase in λ-DNA-coated colloids.
A finite-cluster phase in λ-DNA-coated colloids.
复制标题
λ-DNA 包被的胶体中的有限簇相。
作者：
Tatiana Schmatko;B. Bozorgui;N. Geerts;D. Frenkel;E. Eiser;W. Poon
我们研究了由轮廓长度为 32 µm 的 DNA 桥接的 1 µm 胶体的聚集。我们将两种颗粒与嫁接的双链 λ-DNA 混合，显示短的、互补的单链“突出端”作为自由结合端。共焦显微镜显示形成了稳定的、尺寸有限的簇，其中两种颗粒处于接触接触状态。模拟表明，观察到的紧密接触和生长限制都是由于桥接 DNA 被熵排除在附近颗粒表面之间的空间而造成的。
We studied the aggregation of 1 µm colloids bridged by DNA with 32 µm contour length. We mixed two species of particles with grafted double-stranded λ-DNA displaying short, complementary single-stranded 'overhangs' as free binding-ends. Confocal microscopy showed the formation of stable, size-limited clusters in which the two species of particles were at touching contact. Simulations suggest that the observed close contact and the limitation to grow both result from entropic exclusion of the bridging DNA from the space between nearby particle surfaces.
DOI：
10.1073/pnas.94.12.6185
发表时间：
1997-06-10
影响因子：
11.1
作者：
Baumann, CG;Smith, SB;Bustamante, C
通讯作者：
Bustamante, C