A selective, cell-permeable optical probe for hydrogen peroxide in living cells
A selective, cell-permeable optical probe for hydrogen peroxide in living cells
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DOI：
10.1021/ja0441716
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发表时间：
2004-12-01
影响因子：
15
通讯作者：
Chang, CJ
中科院分区：
文献类型：
--
作者：
Chang, MCY;Pralle, A;Chang, CJ
我们介绍了Peroxyfluor-1（PF 1）的合成，性质和生物学应用，一种新型的光学探针，用于活生物样品中过氧化氢的细胞内成像。PF 1利用硼酸脱保护机制，提供前所未有的选择性和光学动态范围检测过氧化氢在水溶液中类似的活性氧物种，包括超氧化物，一氧化氮，叔丁基过氧化氢，和羟基自由基。我们进一步证明了这种试剂的生物学应用的价值，通过成像的变化[H2 O2]在活的哺乳动物细胞。
We present the synthesis, properties, and biological applications of Peroxyfluor-1 (PF1), a new type of optical probe for intracellular imaging of hydrogen peroxide in living biological samples. PF1 utilizes a boronate deprotection mechanism to provide unprecedented selectivity and optical dynamic range for detecting H2O2in aqueous solution over similar reactive oxygen species including superoxide, nitric oxide,tert-butyl hydroperoxide, and hydroxyl radical. We further demonstrate the value of this reagent for biological applications by imaging changes in [H2O2] in living mammalian cells.