CYCLIN-E CDK2 AND CYCLIN-A CDK2 KINASES ASSOCIATE WITH P107 AND E2F IN A TEMPORALLY DISTINCT MANNER
CYCLIN-E CDK2 AND CYCLIN-A CDK2 KINASES ASSOCIATE WITH P107 AND E2F IN A TEMPORALLY DISTINCT MANNER
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DOI：
10.1101/gad.6.10.1874
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发表时间：
1992-10-01
影响因子：
10.5
通讯作者：
HARLOW, E
中科院分区：
文献类型：
--
作者：
LEES, E;FAHA, B;HARLOW, E
基于其mRNA表达的循环模式，细胞周期蛋白E被分类为推定的G1细胞周期蛋白，在G1/S边界附近检测到最大水平。我们在这里报告，细胞周期蛋白E被发现与转录因子E2 F在一个时间调节的方式。已知E2 F是一个关键的转录因子，用于表达一些S期特异性蛋白，并被认为是一系列其他重要的。细胞周期蛋白E的特异性抗血清被提出，并用于证明细胞周期蛋白E和E2 F之间的关联。这种细胞周期蛋白E/E2 F复合物见于各种组织的各种人类细胞系中，但其出现主要是在细胞周期的G1期检测到的。细胞周期蛋白E/E2 F的关联随着细胞进入S期而减少，就像E2 F与细胞周期蛋白A的关联变得可检测到一样。我们进一步表征了细胞周期蛋白E-E2 F复合物，表明细胞周期蛋白依赖性激酶2（cdk 2）和p107都存在。因此，p107/E2 F复合物与两种不同的cdk 2激酶复合物相关，一种含有细胞周期蛋白A，另一种含有细胞周期蛋白E，这些复合物的出现在细胞周期中受到时间调控。细胞周期蛋白E/E2 F复合物在G1期的存在表明细胞周期蛋白E在控制G1到S过渡所需的基因中的作用。
Cyclin E is classified as a putative G1 cyclin on the basis of its cyclic pattern of mRNA expression, with maximal levels being detected near the G1/S boundary. We report here that cyclin E is found associated with the transcription factor E2F in a temporally regulated fashion. E2F is known to be a critical transcription factor for the expression of some S phase-specific proteins and is thought to be important for a series of others. Antisera specific for cyclin E were raised and used to demonstrate an association between cyclin E and E2F. This cyclin E/E2F complex was seen in a variety of human cell lines from various tissues, but its appearance was detected primarily during the G1 phase of the cell cycle. The cyclin E/E2F association decreased as cells entered S phase, just as the association of E2F with cyclin A became detectable. We characterized the cyclin E-E2F complex further to show that both the cyclin-dependent kinase-2 (cdk2) and p107 were present. Therefore, the p107/E2F complex is associated with two different cdk2 kinase complexes-one containing cyclin A and the other containing cyclin E-and the appearance of these complexes is temporally regulated during the cell cycle. The presence of cyclin E/E2F complexes in the G1 phase suggests a role for cyclin E in the control of genes required for the G1-to-S transition.