Genetic diversity among soybean accessions from three countries measured by RAPDs
Genetic diversity among soybean accessions from three countries measured by RAPDs
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DOI：
10.2135/cropsci2001.4141337x
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发表时间：
2001-07-01
期刊：
影响因子：
2.3
通讯作者：
Nelson, RL
中科院分区：
文献类型：
--
作者：
Li, ZL;Nelson, RL
大豆[Glycine mar（L.）梅尔。]在中国被驯化，但在朝鲜半岛和日本有很长的栽培历史。这三个地区都被认为是大豆种质的重要来源。本研究的目的是评估中国大豆、S。中国8份、韩国3份、日本120份材料进行了遗传多样性分析。韩国各省和三个日本地区;并与遗传多样性模式的地理区域。利用115个随机扩增多态性DNA（RAPD）标记，以欧氏距离表示简单匹配系数，估计遗传关系。采用系统聚类分析、非系统聚类分析和主成分分析确定基因型间的关系。结果表明，中国群体间的平均遗传距离远大于日本和韩国群体间的遗传距离。朝鲜之间的距离小于中日之间和南朝鲜之间的距离。韩国聚类分析和主成分分析几乎完全将中国种质与日本、美国种质分离开来。韩国的品种，但不能区分来自日本和韩国的品种。韩国这些结果与以前利用酶和形态学数据对亚洲大豆种质进行分类的研究一致。聚类分析主要依据RAPD片段在材料间的频率进行聚类，聚类结果基本反映了材料的地理来源。没有明确的关系，发现纬度和遗传多样性之间的加入，从这些国家。虽然日本和S.这些数据表明，目前来自日本和韩国的材料来源于中国。韩国人在基因上与中国人非常不同，但彼此之间更相似。
Soybean [Glycine mar (L.) Merr.] was domesticated in China but has a long history of cultivation on the Korean peninsula and in Japan. All three areas are considered important sources of soybean germplasm. The objectives of this study were to evaluate the genetic variation in soybean within and among China, S. Korea, and Japan by means of 120 accessions from eight Chinese and three S. Korean provinces, and three Japanese districts; and to relate genetic diversity patterns to geographical regions. Genetic relationships were estimated by 115 random amplified polymorphic DNA (RAPD) markers with simple matching coefficients expressed as Euclidean distances. Hierarchical and nonhierarchical cluster analyses as well as principal component analysis were used to define relationships among the genotypes. The results indicate that the mean genetic distance within China is much larger than that within Japan or S. Korea, but smaller than that between China and Japan or S. Korea. Cluster and principal component analyses almost completely separated the accessions from China from those of Japan and S. Korea, but could not distinguish between the accessions from Japan and S. Korea. These results are consistent with previous research using enzymes and morphological data to classify soybean germplasm from Asia. The groups formed by cluster analysis were mainly based on the frequencies of RAPD fragments among accessions and generally reflected the geographical regions of origin. No clear relationship was found between latitude and genetic diversity among accessions from these countries. Although the soybean accessions from Japan and S. Korea originally came from China, these data indicate that current accessions from Japan and S. Korea are genetically very distinct from those from China and more similar to each other.