The role of interstitial Cajal-like cells in the formation of cholesterol stones in guinea pig gallbladder
The role of interstitial Cajal-like cells in the formation of cholesterol stones in guinea pig gallbladder
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DOI：
10.1007/s12072-015-9623-3
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发表时间：
2015-10-01
影响因子：
6.6
通讯作者：
Wang, Xinpeng
中科院分区：
文献类型：
--
作者：
Fan, Ying;Wu, Shuodong;Wang, Xinpeng
探讨间质cajal样细胞（ICLCs）对胆囊肌条收缩的影响；分析豚鼠胆固醇胆石形成过程中iclc的变化。采用高胆固醇饲料（HCD）喂养豚鼠，制备高胆固醇胆结石动物模型。制备离体胆囊肌条。通过CCK-8的慢波收缩频率和幅值评价胆囊运动。免疫组织化学法测定ICLC密度变化。检测c-kit和干细胞因子（stem cell factor， SCF）的表达。iclc受损的胆囊肌条慢波振幅和频率明显降低。与CCK-8收缩反应减弱有关。在HCD豚鼠中，CCK-8对ICLC密度和胆汁流量的影响显著降低。结果表明，胆囊iclc可产生慢波电位，并参与CCK-8诱导的胆囊平滑肌运动的调控。在胆固醇胆结石形成过程中，ICLC密度明显降低。这进一步损害了胆囊的运动能力。ICLC密度的降低可能是由于c-kit和SCF在胆固醇胆结石形成过程中的表达减少所致。
To investigate the effect of interstitial Cajal-like cells (ICLCs) on contraction of gallbladder muscle strips; and to analyze the changes of ICLCs during cholesterol gallstone formation in guinea pig.The cholesterol gallstone animal model was made by feeding guinea pig with high cholesterol diet (HCD). In vitro isolated gallbladder muscle strips were prepared. Gallbladder motility was assessed by the contraction frequency and amplitude of slow wave in response to CCK-8. The alteration in ICLC density was estimated by using immunohistochemistry. The expression of c-kit and stem cell factor (SCF) were determined.The amplitude and frequency of slow wave was significantly lower in gallbladder muscle strips with the impaired ICLCs. And it is correlated with the decreased contractile response to CCK-8. In HCD guinea pig, the ICLC density and bile flow in response to CCK-8 were remarkably decreased. The results indicated that gallbladder ICLCs can create slow wave potential, and also get involved in the regulation of CCK-8 induced gallbladder smooth muscle motility. In the process of cholesterol gallstone formation, ICLC density clearly decreased. This further impaired gallbladder motility. The decrease in ICLC density may result from decreased expression of c-kit and SCF during cholesterol gallstone formation.