Identification and functional analysis of senescence-associated genes in wheat
Identification and functional analysis of senescence-associated genes in wheat
复制标题
小麦衰老相关基因的鉴定及功能分析
DOI：
10.1007/978-1-4939-7672-0_19
复制
发表时间：
2018
影响因子：
--
通讯作者：
Zhou Chunjiang
中科院分区：
文献类型：
--
作者：
Wang Geng;Li Ke;Zhou Chunjiang
衰老是叶片发育的最后阶段。衰老相关基因（Senescence-associated gene，SAGs）在这一过程中发挥重要作用。SAG基因的鉴定和鉴定是阐明这些基因功能和叶片衰老机制的第一步。利用基因芯片、RNA-seq、数字RNA表达水平数据等大量基因组水平的转录组数据筛选候选基因是鉴定SAG的最有效方法之一。在本章中，我们概述了通过微阵列分析鉴定和表征小麦SAGs的方法。还描述了通过筛选转基因植物进行基因功能分析的方法。本章中描述的方案也可以用于其他植物物种，特别是禾本科植物。
Senescence is the final stage of leaf development. During this process, different macromolecules undergo degradation, and the resulting components are transported to developing and storage tissues of the plant.Senescence-associated genes(SAGs) play important roles in this process. Identification and characterization ofSAGsare the first steps to interpret the function of these genes and to elucidate the mechanisms of leaf senescence. One of the most effective ways to identifySAGsis to screen for candidate genes using massive genome-scale transcriptomic data such as microarray, RNA-seq, digital RNA expression level data, etc. The basic functional analysis of candidate genes is to observe the phenotypes of transgenic plants, in which the candidateSAGsare overexpressed, knocked down, or knocked out. In this chapter, we outline methods for identifying and characterizingSAGsby microarray analysis in wheat. Methods of gene functional analyses by screening transgenic plants are also described. The protocols described in this chapter could also be used in other plant species, especially for Poaceae plants.