Nanocapsules incorporating IgG Fc-binding domain derived from Staphylococcus aureus protein A for displaying IgGs on immunosensor chips
Nanocapsules incorporating IgG Fc-binding domain derived from Staphylococcus aureus protein A for displaying IgGs on immunosensor chips
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DOI：
10.1016/j.biomaterials.2010.10.057
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发表时间：
2011-02-01
期刊：
影响因子：
14
通讯作者：
Kuroda, Shun'ichi
中科院分区：
文献类型：
--
作者：
Iijima, Masumi;Kadoya, Hiroyasu;Kuroda, Shun'ichi
为了提高免疫传感器的灵敏度和抗原结合能力，将抗体定向固定在传感器芯片的表面上是至关重要的，但迄今为止，这还没有充分实现。我们描述了一种通过来自金黄色葡萄球菌蛋白A（ZZ-BNC）的IgG Fc结合结构域将免疫球蛋白（IG）吸附到32-nm生物纳米胶囊（BNC）上的方法。这种排列允许类似于60个分子的小鼠总IgG结合到ZZ-BNC，并且所有IgG Fv区向外展示以有效结合抗原。在石英晶体微天平（QCM）传感器芯片的金表面吸附ZZ-BNC可显著提高芯片的灵敏度和抗原结合能力。在表面等离子体共振（SPR）传感器芯片上，ZZ-BNC上的抗体对每种抗原的亲和力均高于蛋白A上的抗体。BNC包被的传感器芯片是非常稳定的，并应证明适用于各种免疫传感器的应用，由于定向固定的抗体。(C)2010爱思唯尔有限公司保留所有权利。
To enhance the sensitivities and antigen-binding capacities of immunosensors, oriented immobilization of antibodies on the surface of the sensor chip is critical, but to date, this has not been adequately achieved. We describe a way of adsorbing immunoglobulin (Ig) proteins onto 32-nm bio-nanocapsules (BNCs) through IgG Fc-binding domains derived from Staphylococcus aureus protein A (ZZ-BNC). This arrangement permits similar to 60 molecules of mouse total IgG bind to ZZ-BNC and all the IgG Fv regions to be displayed outwardly for the effective binding of antigens. ZZ-BNCs adsorbed onto the gold surface of the sensor chip of the quartz crystal microbalance (QCM) could markedly enhance the sensitivity and antigen-binding capacity of the chip. On the sensor chip of surface plasmon resonance (SPR), antibodies on the ZZ-BNCs showed higher affinities to each antigen than those on protein A. The BNC-coated sensor chip is very stable, and should prove useful for various immunosensor applications due to oriented immobilization of antibodies. (C) 2010 Elsevier Ltd. All rights reserved.