Molecular and morphologic characterization of a Cryptosporidium genotype identified in lemurs
Molecular and morphologic characterization of a Cryptosporidium genotype identified in lemurs
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DOI：
10.1016/s0304-4017(02)00384-9
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发表时间：
2003-02-27
影响因子：
2.6
通讯作者：
Eberhard, ML
中科院分区：
文献类型：
--
作者：
da Silva, AJ;Cacciò, S;Eberhard, ML
本研究报道了一种隐孢子虫的分子和形态特征，发现于圈养的狐猴Propithecus verreauxi coquereli的粪便中。从7只出现腹泻的动物（n = 7）中收集粪便样本。经改良抗酸技术染色的粪便涂片的双视野光学显微镜检查显示，在分析的4份粪便样本中存在隐孢子虫属卵囊。所有显微镜下阳性样本均通过PCR确认，使用设计用于扩增来自两个独立位点（即隐孢子虫卵囊壁蛋白（COWP）基因和小亚基核糖体RNA（ssrRNA）基因）的DNA片段的引物。基于全长ssrRNA基因的系统发育分析将该分离株置于包含所有目前已知的C parvum物种/基因型的进化枝内，与C parvum猪基因型密切相关。与GenBank（TM）中的部分ssrRNA序列比较，显示与先前在加拿大患者中鉴定的基因型100%序列相同。通过比较COWP基因部分序列进一步证实了这一发现。出版社：Elsevier Science B. V.
This study reports the molecular and morphologic characterization of a Cryptosporidium sp., identified in stools of captive lemurs Propithecus verreauxi coquereli. Stool samples were collected from seven animals (n = 7) presenting episodes of diarrhea. Bright-field light microscopy of stool smears stained with modified acid-fast technique revealed the presence of Cryptosporidium sp. oocysts in four of the stool samples analyzed. All microscopically positive samples were confirmed by PCR using primers designed to amplify DNA fragments from two independent loci, i.e. the Cryptosporidium oocyst wall protein (COWP) gene and the small subunit ribosomal RNA (ssrRNA) gene. Phylogenetic analysis based on the full-length ssrRNA gene placed this isolate within a clade that contains all currently known C parvum species/genotypes, closely related to the C parvum pig genotype. Comparison with partial ssrRNA sequences available in the GenBank(TM) revealed 100% sequence identity with the genotype previously identified in Canadian patients. This finding was confirmed further by comparison of the COWP gene partial sequences. Published by Elsevier Science B.V.