Genetic analysis of crawling and swimming locomotory patterns in C. elegans
Genetic analysis of crawling and swimming locomotory patterns in C. elegans
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DOI：
10.1073/pnas.0810359105
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发表时间：
2008-12-30
影响因子：
11.1
通讯作者：
McIntire, Steven L.
中科院分区：
文献类型：
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作者：
Pierce-Shimomura, Jonathan T.;Chen, Beth L.;McIntire, Steven L.
在无脊椎动物和哺乳动物中，神经活动的替代模式驱动不同的节奏运动模式。负责节律性行为模式表达的神经分子机制知之甚少。在这里，我们表明，秀丽隐杆线虫切换不同形式的运动，或爬行与游泳，固体和液体环境之间的过渡时。这些运动形式的区别在于不同的运动学和不同的神经肌肉活动的潜在模式，如体内钙成像所确定的。游泳与爬行节律的表达受感觉输入的调节。在筛选爬行和游泳行为之间的转换有缺陷的突变体时，我们鉴定了unc-79和unc-80，这两种突变体已知在NCA离子通道稳定性方面有缺陷。遗传和行为分析表明，NCA通道使C。优雅的。unc-79、unc-80和NCA通道代表了一组保守的对行为模式产生至关重要的基因。
Alternative patterns of neural activity drive different rhythmic locomotory patterns in both invertebrates and mammals. The neuro-molecular mechanisms responsible for the expression of rhythmic behavioral patterns are poorly understood. Here we show that Caenorhabditis elegans switches between distinct forms of locomotion, or crawling versus swimming, when transitioning between solid and liquid environments. These forms of locomotion are distinguished by distinct kinematics and different underlying patterns of neuromuscular activity, as determined by in vivo calcium imaging. The expression of swimming versus crawling rhythms is regulated by sensory input. In a screen for mutants that are defective in transitioning between crawl and swim behavior, we identified unc-79 and unc-80, two mutants known to be defective in NCA ion channel stabilization. Genetic and behavioral analyses suggest that the NCA channels enable the transition to rapid rhythmic behaviors in C. elegans. unc-79, unc-80, and the NCA channels represent a conserved set of genes critical for behavioral pattern generation.