Covalent binding to apoprotein is a major fate of heme in a variety of reactions in which cytochrome P-450 is destroyed.
Covalent binding to apoprotein is a major fate of heme in a variety of reactions in which cytochrome P-450 is destroyed.
复制标题
在细胞色素 P-450 被破坏的各种反应中，与脱辅基蛋白的共价结合是血红素的主要命运。
DOI：
10.1016/0006-291x(86)90265-2
复制
发表时间：
1986
影响因子：
3.1
通讯作者：
Guengerich,FP
中科院分区：
文献类型：
--
作者：
Guengerich,FP
用~(14)C或~(3)H标记大鼠肝微体细胞色素P-450中的血红素，并在体外与多种已知破坏细胞色素P-450的试剂孵育后进行分级。在所有情况下，包括与氟、1-辛烯、溴乙烯、三氯乙烯、氯乙烯、对硫磷、过氧化异丙苯、NaN3或铁-ADP络合物孵育，血红素标记的主要部分都不可逆地与载脂蛋白结合。当纯化和重组的细胞色素P-450与NADPH和氯乙烯孵育时，LABEL也广泛结合到载脂蛋白上。这一过程似乎是广泛的，并在很大程度上参与了许多化合物对细胞色素P-450血红素的破坏。
The heme in rat liver microsomal cytochrome P-450 was labeled with 14 C or 3 H and the microsomes were fractionated after in vitro incubations with a variety of agents known to destroy cytochrome P-450 heme. A major fraction of the heme label was irreversibly bound to apoprotein in all cases, including incubations with fluroxene, 1-octene, vinyl bromide, trichloroethylene, vinyl chloride, parathion, cumene hydroperoxide, NaN 3, or iron-ADP complex. Label was also extensively bound to apoprotein when purified and reconstituted cytochrome P-450 was incubated with NADPH and vinyl chloride. This process appears to be widespread and involved to a significant extent in the cytochrome P-450 heme destruction observed with many compounds.