Enzymatic Reactions using Ionic Liquids for Green Sustainable Chemical Process; Stabilization and Activation of Lipases
Enzymatic Reactions using Ionic Liquids for Green Sustainable Chemical Process; Stabilization and Activation of Lipases
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使用离子液体的酶促反应实现绿色可持续化学工艺；
DOI：
10.1002/tcr.202200275
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发表时间：
2023
期刊：
影响因子：
--
通讯作者：
T.
中科院分区：
文献类型：
--
作者：
Itoh;T.
从环境友好的角度来看，酶反应受到高度尊重。在努力开发新的酶的同时，必须研究酶的反应介质和支持材料的优化。脂肪酶由于具有广泛的底物、稳定性和可用性，经常被用作合成工具，甚至工业上的合成工具。我们研究了ILS既作为溶剂又作为酶的活化剂或稳定剂的可能性，特别是作为模型酶的脂肪酶。ILS使脂肪酶可循环使用，显著加速了酯交换反应，并通过冷冻干燥过程固定化提高了脂肪酶的稳定性和反应活性。我们讨论了如何利用以脂肪酶催化的反应为中心的IL工程来提高酶的性能，即 ，即实现酶的可循环使用，ILS如何介导提高反应速度，以及提高酶的稳定性。
The enzymatic reaction is highly respected from an environmentally‐friendly point‐of‐view. Optimization of the reaction media and supporting materials of enzymes must be investigated in parallel with the effort to develop new enzymes. Lipases are frequently used for organic syntheses as synthetic tools even industry because of their acceptance of having a broad range of substrates, stability, and availability. We have investigated the possibility of ILs as both a solvent and activating or stabilization agent of enzymes, in particular, lipase as a model enzyme. ILs allowed recyclable use of a lipase and significant acceleration of transesterification, and also enhanced the stability and reaction activity of a lipase by immobilization through a lyophilization process. We discuss how we enhanced the enzyme capability using the IL engineering focusing on lipase‐catalyzed reactions, i. e., realization of the recyclable use of an enzyme, how ILs mediated the enhanced reaction rate, and improved the stability of the enzyme.