Imaging Correlates of Differential Expression of Indoleamine 2,3-Dioxygenase in Human Brain Tumors
Imaging Correlates of Differential Expression of Indoleamine 2,3-Dioxygenase in Human Brain Tumors
复制标题
DOI：
10.1007/s11307-009-0225-0
复制
发表时间：
2009-11-01
影响因子：
3.1
通讯作者：
Chugani, Diane C.
中科院分区：
文献类型：
--
作者：
Batista, Carlos E. A.;Juhasz, Csaba;Chugani, Diane C.
背景：由吲哚胺 2,3-双加氧酶 (IDO) 介导的犬尿氨酸途径的色氨酸分解代谢是肿瘤免疫抵抗的一种机制。使用 α-[C-11]甲基-L-色氨酸 (AMT) 的正电子发射断层扫描 (PET) 可以量化浸润性胶质瘤和胶质神经元肿瘤中色氨酸的转运和代谢。在本研究中，我们研究了 PET 测量的脑肿瘤中色氨酸代谢增加是否与切除的脑肿瘤标本中 IDO 的表达有关。方法：通过免疫组织化学方法评估了 15 名原发性脑肿瘤患者（中位年龄为 34 岁）的肿瘤标本中的 IDO 表达，这些患者在肿瘤切除前接受了 AMT PET 扫描。比较低级别和高级别肿瘤之间的 IDO 表达模式，以及 PET 测量的 AMT 转运和代谢。结果：在 7 个低级别肿瘤中的 6 个肿瘤细胞中观察到 IDO 免疫反应性，但仅在 8 个高级别肿瘤中的 1 个中观察到 IDO 免疫反应性 (p=0.01)；后面的三个肿瘤仅显示内皮染色。低度肿瘤表现出较低的转运率（p
Background: Tryptophan catabolism via the kynurenine pathway, mediated by indoleamine 2,3-dioxygenase (IDO), is a mechanism involved in tumor immunoresistance. Positron emission tomography (PET) with alpha-[C-11]methyl-L-tryptophan (AMT) can quantify transport and metabolism of tryptophan in infiltrating gliomas and glioneuronal tumors. In the present study, we investigated whether increased tryptophan metabolism in brain tumors measured by PET is related to expression of IDO in resected brain tumor specimens.Methods: IDO expression was assessed by immunohistochemistry in tumor specimens from 15 patients ( median age, 34 years) with primary brain tumors who underwent AMT PET scanning before tumor resection. Patterns of IDO expression were compared between low- and high-grade tumors and also to AMT transport and metabolism measured on PET.Results: IDO immunoreactivity was seen in tumor cells in six of seven low- grade tumors but only in one of eight high-grade tumors (p=0.01); three of these latter tumors showed endothelial staining only. Low-grade neoplasms showed lower transport rate (p