Characterization of the first cultured representative of a Bacteroidetes clade specialized on the scavenging of cyanobacteria
Characterization of the first cultured representative of a Bacteroidetes clade specialized on the scavenging of cyanobacteria
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DOI：
10.1111/1462-2920.13639
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发表时间：
2017-03-01
影响因子：
5.1
通讯作者：
Spring, Stefan
中科院分区：
文献类型：
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作者：
Ben Hania, Wajdi;Joseph, Manon;Spring, Stefan
厌氧、中温和中度嗜盐菌株L21-Spi-D4(T)最近用富含蛋白质的Arthrospira（原螺旋藻）platensis提取物作为底物从高盐蓝藻垫的缺氧区分离出来。基于16S rRNA基因的系统发育分析表明，该菌株与拟杆菌门分支MgMjR-022有亲缘关系，该分支广泛分布于高盐微生物席中，迄今为止仅包含未培养的细菌序列。对菌株L21-Spi-D4(T)全基因组序列的分析表明，该菌株可能具有降解蓝藻生物量的特化功能。除了降解特定蓝藻蛋白的酶基因外，还发现了一个明显的多肽蓝藻素转运复合体，该复合体与拟杆菌门的典型多糖利用位点同源。MgMjR-022分支的环境16S rRNA基因序列与高盐垫缺氧区蓝藻的共同出现模式明显且可重复，表明该分支的成员对腐烂的蓝藻具有特定的依赖性。在表型、基因组和生态学特征比较分析的基础上，我们提出建立以L21-Spi-D4(T)和Salinivirgaceae fam为代表的新分类群Salinivirga cyanobacteriivorans gen. nov., sp. nov.。11月，包含MgMjR-022进化支的序列。
The anaerobic, mesophilic and moderately halophilic strain L21-Spi-D4(T) was recently isolated from the suboxic zone of a hypersaline cyanobacterial mat using protein-rich extracts of Arthrospira (formerly Spirulina) platensis as substrate. Phylogenetic analyses based on 16S rRNA genes indicated an affiliation of the novel strain with the Bacteroidetes clade MgMjR-022, which is widely distributed and abundant in hypersaline microbial mats and heretofore comprised only sequences of uncultured bacteria. Analyses of the complete genome sequence of strain L21-Spi-D4(T) revealed a possible specialization on the degradation of cyanobacterial biomass. Besides genes for enzymes degrading specific cyanobacterial proteins a conspicuous transport complex for the polypeptide cyanophycin could be identified that is homologous to typical polysaccharide utilization loci of Bacteroidetes. A distinct and reproducible co-occurrence pattern of environmental 16S rRNA gene sequences of the MgMjR-022 clade and cyanobacteria in the suboxic zone of hypersaline mats points to a specific dependence of members of this clade on decaying cyanobacteria. Based on a comparative analysis of phenotypic, genomic and ecological characteristics we propose to establish the novel taxa Salinivirga cyanobacteriivorans gen. nov., sp. nov., represented by the type strain L21-Spi-D4(T), and Salinivirgaceae fam. nov., comprising sequences of the MgMjR-022 clade.