X-ray structure of T4 endonuclease V: an excision repair enzyme specific for a pyrimidine dimer.
X-ray structure of T4 endonuclease V: an excision repair enzyme specific for a pyrimidine dimer.
复制标题
T4 核酸内切酶 V 的 X 射线结构：一种嘧啶二聚体特异性切除修复酶。
作者：
Kosuke Morikawa;Osamu Matsumoto;Michiko Tsujimoto;K. Katayanagi;M. Ariyoshi;Takefumi Doi;Morio Ikehara;Tetsuya Inaoka;Eiko Ohtsuka
T4内切酶V（一种负责嘧啶-二聚体特异性切除-修复途径第一步的酶）的x射线结构在1.6埃分辨率下被确定。该酶由一个紧凑的结构域组成，该结构域被划分为全α结构。这个单一结构域有两种不同的催化活性；它的功能是嘧啶二聚体糖基化酶和无嘌呤-无嘧啶内切酶。氨基末端段穿透在两个主螺旋之间，阻止它们直接接触。精细的结构表明残基参与了底物结合和糖基化反应的催化作用。
The x-ray structure of T4 endonuclease V, an enzyme responsible for the first step of a pyrimidine-dimer-specific excision-repair pathway, was determined at a 1.6-angstrom resolution. The enzyme consists of a single compact domain classified into an all-alpha structure. This single domain has two distinct catalytic activities; it functions as a pyrimidine dimer glycosylase and as an apurinic-apyrimidinic endonuclease. The amino-terminal segment penetrates between two major helices and prevents their direct contact. The refined structure suggests the residues involved in the substrate binding and the catalysis of the glycosylation reaction.
噬菌体 T4 和藤黄微球菌 UV 特异性核酸内切酶切割嘧啶二聚体的比较。
DOI：
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发表时间：
1980
期刊：
The Journal of biological chemistry
影响因子：
--
作者：
Gordon,LK;Haseltine,WA
通讯作者：
Haseltine,WA