Galectin-1 Secreted by Activated Stellate Cells in Pancreatic Ductal Adenocarcinoma Stroma Promotes Proliferation and Invasion of Pancreatic Cancer Cells An In Vitro Study on the Microenvironment of Pancreatic Ductal Adenocarcinoma
Galectin-1 Secreted by Activated Stellate Cells in Pancreatic Ductal Adenocarcinoma Stroma Promotes Proliferation and Invasion of Pancreatic Cancer Cells An In Vitro Study on the Microenvironment of Pancreatic Ductal Adenocarcinoma
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胰管腺癌基质中活化星状细胞分泌的Galectin-1促进胰腺癌细胞增殖和侵袭 胰管腺癌微环境的体外研究
DOI：
10.1097/mpa.0b013e318217945e
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发表时间：
2011-08-01
期刊：
影响因子：
2.9
通讯作者：
Jiang, Kuirong
中科院分区：
文献类型：
--
作者：
Xue, Xiaofeng;Lu, Zipeng;Jiang, Kuirong
目的：探讨活化的胰腺星状细胞是胰腺导管腺癌组织间质高表达Galectin-1的来源，并探讨其对体外培养的胰腺癌细胞株CFPAC-1增殖和侵袭能力的影响。免疫组织化学、定量聚合酶链式反应和Western印迹法检测PDAC组织中Galectin-1的细胞来源。采用改良的Boyden小室，建立胰腺癌细胞株CFPAC-1体外共培养体系，并在此基础上评价其在Galectin-1拮抗剂作用下的增殖和侵袭能力。结果：PaSCs是PDAC间质中高表达Galectin-1的主要来源。PaSCs分泌的Galectin-1在增殖实验中增加CFPAC-1的增殖率，在侵袭实验中促进CFPAC-1的侵袭。结论：在恶性环境下，PaSCs表达并分泌Galectin-1，从而进一步促进癌细胞的增殖和侵袭。
Objectives: This study aimed to clarify that the activated pancreatic stellate cells (PaSCs) are the origin of the highly expressed galectin-1 in the stroma of pancreatic ductal adenocarcinoma (PDAC) tissue and to evaluate the effect of the secreted galectin-1 on proliferation and invasion ability of pancreatic cancer cell line CFPAC-1 in vitro.Methods: Different kinds of PaSCs were isolated from the normal or cancerous pancreatic tissues and cultured. Immunohistochemistry study, quantitative polymerase chain reaction, and Western blot were carried out to check the cellular origin of galectin-1 in PDAC tissue. By using modified Boyden chambers, in vitro coculture system of PaSCs was established with the pancreatic cancer cell line CFPAC-1 and based on which we assessed the proliferation and invasion ability of CFPAC-1 with or without galectin-1 antagonists.Results: We identified PaSCs as the primary source of the highly expressed galectin-1 in PDAC stroma. Galectin-1 secreted by PaSCs increased CFPAC-1 proliferative rate in the proliferation assay and facilitated CFPAC-1 infiltration in the invasion assay.Conclusions: Under malignant circumstances, PaSCs express and secret galectin-1, which could further promote the proliferation and invasion of cancer cells.