Genome-wide experimental determination of barriers to horizontal gene transfer
Genome-wide experimental determination of barriers to horizontal gene transfer
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DOI：
10.1126/science.1147112
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发表时间：
2007-11-30
期刊：
影响因子：
56.9
通讯作者：
Rubin, Edward M.
中科院分区：
文献类型：
--
作者：
Sorek, Rotem;Zhu, Yiwen;Rubin, Edward M.
水平基因转移，即遗传物质从一个生物体的基因组转移到另一个生物体的基因组，主要通过计算序列分析在微生物物种中进行了研究。为了解决缺乏实验数据的问题，我们研究了来自79个原核生物基因组的246,045个基因试图转移到大肠杆菌中，并确定了始终无法转移的基因。我们通过将不同物种的编码区置于诱导启动子的控制下，研究了转移抑制的机制。我们的数据表明，对宿主的毒性抑制了转移，而与来源物种无关，基因剂量的增加和相关表达的增加可能是转移失败的主要原因。尽管这些实验研究仅检查了大肠杆菌的转移，但对细菌和古细菌基因组的基因转移率的计算分析支持我们研究中观察到的障碍在整个生命树中普遍存在。
Horizontal gene transfer, in which genetic material is transferred from the genome of one organism to that of another, has been investigated in microbial species mainly through computational sequence analyses. To address the lack of experimental data, we studied the attempted movement of 246,045 genes from 79 prokaryotic genomes into Escherichia coli and identified genes that consistently fail to transfer. We studied the mechanisms underlying transfer inhibition by placing coding regions from different species under the control of inducible promoters. Our data suggest that toxicity to the host inhibited transfer regardless of the species of origin and that increased gene dosage and associated increased expression may be a predominant cause for transfer failure. Although these experimental studies examined transfer solely into E. coli, a computational analysis of gene-transfer rates across available bacterial and archaeal genomes supports that the barriers observed in our study are general across the tree of life.