Enzymatic saccharification of cornstalk by onsite cellulases produced by Trichoderma viride for enhanced biohydrogen production
Enzymatic saccharification of cornstalk by onsite cellulases produced by Trichoderma viride for enhanced biohydrogen production
复制标题
利用绿色木霉产生的现场纤维素酶对玉米杆进行酶促糖化，以提高生物氢产量
DOI：
10.1111/gcbb.12022
复制
发表时间：
2013-09-01
影响因子：
5.6
通讯作者：
Ren, Nan-Qi
中科院分区：
文献类型：
--
作者：
Zhao, Lei;Cao, Guang-Li;Ren, Nan-Qi
Lignocellulosic biomass, if properly saccharified, could be an ideal feedstock for biohydrogen production. However, the high cellulases cost is the key obstacle to its development. In this work, cost-effective enzyme produced by Trichoderma viride was used to saccharify cornstalk. To obtain high sugar yield, a central composite design of response surface method was used to optimize enzymatic saccharification process. Experimental results showed that the enzymatic saccharification rate reached the highest of 81.2% when pH, temperature, cellulases and substrate concentration were 5, 49.7 degrees C, 35.7IUg(-1), and 38.5gL(-1), respectively. The cornstalk hydrolysate was subsequently introduced to fermentation by Thermoanaerobacterium thermosaccharolyticum W16, the yield of hydrogen reached the highest level of 90.6ml H(2)g(-1) pretreated cornstalk. The present results indicate the potential of using T.thermosaccharolyticum W16 for high yield conversion of cornstalk hydrolysate, which was saccharified by onsite enzyme produced by T.viride.
