Normal pregnancy and preeclampsia both produce inflammatory changes in peripheral blood leukocytes akin to those of sepsis
Normal pregnancy and preeclampsia both produce inflammatory changes in peripheral blood leukocytes akin to those of sepsis
复制标题
DOI：
10.1016/s0002-9378(98)70254-6
复制
发表时间：
1998-07-01
影响因子：
9.8
通讯作者：
Redman, CWG
中科院分区：
文献类型：
--
作者：
Sacks, GP;Studena, K;Redman, CWG
目的：我们的目的是寻找子痫前期循环白细胞活化的证据。研究设计：采用全血流式细胞技术分析活化表面标志物（CD11b、CD14、CD23、CD49d、CD62L、CD64、CD66b、HLA-DR）和细胞内活性氧。样本取自21名先兆子痫妇女、21名匹配的正常孕妇、21名健康的非孕妇对照和6名非孕妇败血症患者。10例子痫前期患者于产后6周随访。结果：健康孕妇的白细胞与非孕妇有显著性差异（CD11b、CD14和CD64增加，细胞内活性氧增加）。在子痫前期，除了这些变化外，l -选择素的表达减少，细胞内活性氧进一步增加。在正常妊娠和子痫前期发现的变化与败血症相似，但不完全相同。结论：正常妊娠晚期以外周血白细胞显著活化为特征，且在子痫前期进一步升高。
OBJECTIVE: Our aim was to seek evidence for circulating leukocyte activation in preeclampsia.STUDY DESIGN: Whole blood flow cytometric techniques were used to analyze surface markers of activation (CD11b, CD14, CD23, CD49d, CD62L, CD64, CD66b, HLA-DR) and intracellular reactive oxygen species. Samples were taken from 21 women with preeclampsia, 21 matched normal pregnant women, 21 healthy nonpregnant controls, and 6 nonpregnant patients with septicemia. Ten preeclamptic cases were followed up 6 weeks post partum.RESULTS: The leukocytes of healthy pregnant women differed substantially and significantly from those of nonpregnant women (increased CD11b, CD14, and CD64 and increased intracellular reactive oxygen species). In preeclampsia there was, in addition to these changes, reduced expression of L-selectin and further increases in intracellular reactive oxygen species. The changes found in normal pregnancy and preeclampsia were similar, but not identical, to those found in sepsis.CONCLUSIONS: Normal third-trimester pregnancy is characterized by remarkable activation of peripheral blood leukocytes, which is further increased in preeclampsia.