Using FRET to measure the time it takes for a cell to destroy a virus
Using FRET to measure the time it takes for a cell to destroy a virus
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DOI：
10.1039/c9nr09816j
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发表时间：
2020-04-28
期刊：
影响因子：
6.7
通讯作者：
Gassensmith, Jeremiah J.
中科院分区：
文献类型：
--
作者：
Benjamin, Candace E.;Chen, Zhuo;Gassensmith, Jeremiah J.
在过去的二十年里，病毒纳米技术的出现创造了许多诱人的可能的翻译应用；然而，病毒纳米颗粒在细胞中的玻璃酸盐仍然是一个悬而未决的问题。在这里，我们研究了病毒样颗粒(VLP)Qβ的稳定性和寿命--一种具有代表性的和流行的VLP在几种应用中的应用--跟随细胞摄取。通过利用病毒表面可用的功能手柄，我们已经正交安装了已知的FRET对，FITC和罗丹明B，以了解颗粒在体外的行为。根据这些数据，我们认为血管内皮细胞在细胞摄取后几个小时内除了预期的蛋白分解外还会发生聚集。
The emergence of viral nanotechnology over the preceding two decades has created a number of intellectually captivating possible translational applications; however, thein vitrofate of the viral nanoparticles in cells remains an open question. Herein, we investigate the stability and lifetime of virus-like particle (VLP) Q beta-a representative and popular VLP for several applications-following cellular uptake. By exploiting the available functional handles on the viral surface, we have orthogonally installed the known FRET pair, FITC and Rhodamine B, to gain insight of the particle's behaviorin vitro. Based on these data, we believe VLPs undergo aggregation in addition to the anticipated proteolysis within a few hours of cellular uptake.