Effect of chronic ethanol exposure on female rat reproductive cyclicity and hormone secretion
Effect of chronic ethanol exposure on female rat reproductive cyclicity and hormone secretion
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DOI：
10.1097/00000374-200107000-00012
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发表时间：
2001-07-01
影响因子：
3.2
通讯作者：
Emanuele, MA
中科院分区：
文献类型：
--
作者：
Emanuele, NV;LaPaglia, N;Emanuele, MA
背景：乙醇暴露会损害哺乳动物的生殖功能。然而，其机制尚不完全清楚。方法：给予成年雌性大鼠乙醇或热量匹配的对照饮食。第三组喂食不含乙醇的液体食物。一半动物在2周（短慢性）时被杀，另一半动物在2个月（长慢性）时被杀，均在发情当天，每天阴道涂片。结果：乙醇饲喂对大鼠的主要影响是扰乱发情周期。虽然所有成对喂养的动物都继续循环，但在短期慢性研究中，40%的乙醇大鼠的周期中断。在长期的慢性研究中，83%的乙醇动物有异常的循环，相比之下，16%的成对喂养的对照组。周期中断的本质是发情的延长，与发情高潮之间的时间间隔增加。在4只喂食乙醇的大鼠中，发情周期完全停止。然而，乙醇没有减少卵巢或子宫的重量。在短期慢性研究中，乙醇显著增加血清雌二醇，而不是长期慢性研究，而黄体酮没有变化。乙醇确实导致循环中的胰岛素样生长因子显著减少。结论：短期慢性和长期慢性乙醇暴露的主要影响是破坏发情规律，导致发情高潮次数减少。这种破坏的部分机制可能是短暂的雌激素增加或循环中的胰岛素样生长因子减少。
Background: Ethanol exposure impairs mammalian reproductive function. However, the mechanisms are not fully understood.Methods: Adult female rats were given an ethanol or a calorically matched control diet. A third group was given a liquid nonethanol diet. Half the animals were killed at 2 weeks (short chronic) and the other half at 2 months (long chronic), all on the day of proestrous on the basis of daily vaginal smears.Results: The major effect of ethanol feeding was disruption in the estrous cycle. Although all of the pair-fed animals continued to cycle, 40% of the ethanol rats in the short chronic study had disruption of their cycles. In the long chronic study, 83% of the ethanol animals had abnormal cycling, in contrast to 16% of the pair-fed controls. The nature of the cycle disruption was prolongation of diestrous, with an increased time interval between proestrous surges. In four ethanol-fed rats, there was complete cessation of the estrous cycle. However, ethanol did not decrease ovarian or uterine weight. Ethanol significantly increased serum estradiol in the short chronic but not long chronic study, whereas progesterone was unchanged. Ethanol did cause a significant reduction in circulating insulin-like growth factor.Conclusions: The major effect of both short chronic and long chronic ethanol exposure was disruption of the estrous regularity, leading to a decreased number of proestrous surges. Part of the mechanism of this disruption might be a transient estrogen increase or a decrease in circulating insulin-like growth factor.