Analysis of proteasomal proteolysis during the in vitro metacyclogenesis of Trypanosoma cruzi.
Analysis of proteasomal proteolysis during the in vitro metacyclogenesis of Trypanosoma cruzi.
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DOI：
10.1371/journal.pone.0021027
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发表时间：
2011
期刊：
影响因子：
3.7
通讯作者：
Krieger MA
中科院分区：
文献类型：
--
作者：
Cardoso J;Lima Cde P;Leal T;Gradia DF;Fragoso SP;Goldenberg S;De Sá RG;Krieger MA
Proteasomes are large protein complexes, whose main function is to degrade unnecessary or damaged proteins. The inhibition of proteasome activity in Trypanosoma cruzi blocks parasite replication and cellular differentiation. We demonstrate that proteasome-dependent proteolysis occurs during the cellular differentiation of T. cruzi from replicative non-infectious epimastigotes to non-replicative and infectious trypomastigotes (metacyclogenesis). No peaks of ubiquitin-mediated degradation were observed and the profile of ubiquitinated conjugates was similar at all stages of differentiation. However, an analysis of carbonylated proteins showed significant variation in oxidized protein levels at the various stages of differentiation and the proteasome inhibition also increased oxidized protein levels. Our data suggest that different proteasome complexes coexist during metacyclogenesis. The 20S proteasome may be free or linked to regulatory particles (PA700, PA26 and PA200), at specific cell sites and the coordinated action of these complexes would make it possible for proteolysis of ubiquitin-tagged proteins and oxidized proteins, to coexist in the cell.
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DOI：
10.1084/jem.184.5.1909
发表时间：
1996-11-01
期刊：
The Journal of experimental medicine
影响因子：
--
作者：
通讯作者：
--
影响因子：
4.8
作者：
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通讯作者：
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影响因子：
4.8
作者：
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通讯作者：
DAVIES, KJA
影响因子：
1.9
作者：
Gutierrez, Bessy;Osorio, Luis;Gonzalez, Jorge
通讯作者：
Gonzalez, Jorge
DOI：
10.1016/s0006-291x(03)00809-x
发表时间：
2003-06-06
影响因子：
3.1
作者：
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通讯作者：
Davies, KJA