Use of reporter genes to study promoters of the androgen receptor.
Use of reporter genes to study promoters of the androgen receptor.
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使用报告基因研究雄激素受体的启动子。
DOI：
10.1007/978-1-60327-378-7_12
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发表时间：
2009
期刊：
影响因子：
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通讯作者：
Shemshedini,Lirim
中科院分区：
文献类型：
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作者：
Shemshedini,Lirim
雄激素受体（AR）作为一种转录调节因子，调节许多基因的表达，这些基因对男性性分化至关重要，包括正常前列腺和前列腺癌的发展。AR通过与在受调节基因的启动子上发现的调节DNA序列结合而起作用。通过使用报告基因测定极大地促进了对这种响应性启动子的AR活性的研究，其提供了用于研究这种启动子的活性的定量和可再现的方法。在可以使用的几种报告基因中，编码荧光素酶（Luc）和氯霉素乙酰转移酶（CAT）的基因已经被对AR调节启动子感兴趣的研究人员最广泛和成功地使用。这些研究已经导致了DNA调控元件介导的AR活性的响应启动子的识别和表征，并提高了对AR如何调节转录过程的理解。本章描述的是一种方法，通过该方法产生和利用Luc和CAT报告基因质粒驱动的一种新的雄激素调节基因，ETV1的启动子。
As a transcriptional regulator, the androgen receptor (AR) regulates the expression of many genes that are essential for male sexual differentiation, including the development of both normal prostate and prostate cancer. The AR acts by binding to regulatory DNA sequences found on the promoters of regulated genes. The study of AR activity on such responsive promoters is greatly facilitated by the use of the reporter gene assay, which provides a quantitative and reproducible method for studying the activity of such promoters. Among the several reporter genes that can be used, the genes encoding luciferase (Luc) and chloramphenicol acetyltransferase (CAT) have been used most widely and successfully by researchers interested in AR-regulated promoters. Such studies have led to the identification and characterization of DNA regulatory elements mediating AR activity on responsive promoters and to an improved understanding of how AR regulates the transcription process. Described in this chapter is a method by which to generate and utilize Luc and CAT reporter gene plasmids driven by the promoter of a novel androgen-regulated gene, ETV1.