Microenvironment Influences Cancer Cell Mechanics from Tumor Growth to Metastasis
Microenvironment Influences Cancer Cell Mechanics from Tumor Growth to Metastasis
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DOI：
10.1007/978-3-319-95294-9_5
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发表时间：
2018-01-01
期刊：
影响因子：
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通讯作者：
Dawson, Michelle R.
中科院分区：
文献类型：
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作者：
Ghosh, Deepraj;Dawson, Michelle R.
实体肿瘤中的微环境包括多种细胞类型、基质蛋白和生长因子，它们通过提供物理和化学刺激来深刻地影响癌细胞力学。肿瘤微环境具有动态和异质性，在肿瘤从原发肿瘤生长到转移和耐药肿瘤的发展过程中起着至关重要的作用。本章概述了用于研究癌细胞力学和肿瘤微环境在癌症进展的不同阶段的力学变化的生物物理工具，包括原发肿瘤的生长、局部侵袭和转移。定量的单细胞生物物理分析细胞内力学、细胞牵引力和细胞运动可以很容易地与关键细胞命运过程的分析相结合，包括粘附、增殖和耐药性，以确定力学变化如何促进癌症进展。这种生物物理方法可用于系统地研究肿瘤中控制癌细胞与基质相互作用的参数，并确定诱导肿瘤促进行为的特定条件，以及抑制这些条件以治疗癌症的策略。增加对驱动癌症进展的潜在生物物理机制的理解可能会为对抗癌症的新治疗方法提供见解。
The microenvironment in a solid tumor includes a multitude of cell types, matrix proteins, and growth factors that profoundly influence cancer cell mechanics by providing both physical and chemical stimulation. This tumor microenvironment, which is both dynamic and heterogeneous in nature, plays a critical role in cancer progression from the growth of the primary tumor to the development of metastatic and drug- resistant tumors. This chapter provides an overview of the biophysical tools used to study cancer cell mechanics and mechanical changes in the tumor microenvironment at different stages of cancer progression, including growth of the primary tumor, local invasion, and metastasis. Quantitative single cell biophysical analysis of intracellular mechanics, cell traction forces, and cell motility can easily be combined with analysis of critical cell fate processes, including adhesion, proliferation, and drug resistance, to determine how changes in mechanics contribute to cancer progression. This biophysical approach can be used to systematically investigate the parameters in the tumor that control cancer cell interactions with the stroma and to identify specific conditions that induce tumor-promoting behavior, along with strategies for inhibiting these conditions to treat cancer. Increased understanding of the underlying biophysical mechanisms that drive cancer progression may provide insight into novel therapeutic approaches in the fight against cancer.