Epigenetic control of defense genes following MeJA-induced priming in rice (O-sativa)
Epigenetic control of defense genes following MeJA-induced priming in rice (O-sativa)
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DOI：
10.1016/j.jplph.2018.06.007
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发表时间：
2018-09-01
影响因子：
4.3
通讯作者：
Caruso, Carla
中科院分区：
文献类型：
--
作者：
Bertini, Laura;Proietti, Silvia;Caruso, Carla
在植物防御中，引发是植物准备更快或更积极地响应未来生物或非生物胁迫的生理过程。这种独特的生理状态可以通过有益微生物或天然或合成化合物的治疗来诱导。引发现象已被大量描述，主要是在双子叶植物中，但对调控这一现象的分子机制的研究仍处于起步阶段。一个有趣的可能性是，表观遗传变化可能是启动建立的主要演员。由于DNA甲基化和/或组蛋白修饰，更容易接近的染色质结构可以促进对随后攻击的更快和更有效的基因反应。在这里，我们研究了茉莉酸甲酯（MeJA）诱导的引发对单子叶植物（水稻）对机械伤害的反应效能的影响。特别是，我们表明，MeJA引物植物增加表达的防御相关基因，如OsBBPI和OsPDX，受伤后。最后，我们提供的证据表明，MeJA调节组蛋白修饰的启动子区域的OsBBPI，以及在全基因组DNA甲基化水平的变化。这些研究共同证实了引发在加强植物防御中的重要性，并支持越来越多的证据表明，表观遗传调控在引发发生中起着关键作用。
In plant defense, priming is a physiological process by which a plant prepares to more quickly or aggressively respond to a future biotic or abiotic stress. This unique physiological state can be induced by beneficial microbes or by treatment with natural or synthetic compounds. Priming has been described copiously, mainly in dicots, but the study on the molecular mechanisms regulating the phenomenon is still at its infancy. A fascinating possibility is that epigenetic changes may be the main actors on priming establishment. A more accessible chromatin structure, due to DNA methylation and/or histone modifications, could facilitate a quicker and more potent gene response to a subsequent attack. Here, we investigated the impact of methyl jasmonate (MeJA)induced priming on the response efficacy to mechanical wounding of a monocot (Oryza sativa). In particular, we showed that MeJA primes plants for increased expression of defense-related genes, such as OsBBPI and OsPDX, upon wounding. Finally, we provided evidence that MeJA modulates histone modifications in the promoter region of OsBBPI, as well as changes at genome-wide DNA methylation level. Together these studies corroborate the importance of priming in strengthening plant defense and support the growing evidence that epigenetic regulation plays a pivotal role in priming onset.