TRANSPOSON-MEDIATED SITE-SPECIFIC RECOMBINATION INVITRO - DNA CLEAVAGE AND PROTEIN-DNA LINKAGE AT THE RECOMBINATION SITE
TRANSPOSON-MEDIATED SITE-SPECIFIC RECOMBINATION INVITRO - DNA CLEAVAGE AND PROTEIN-DNA LINKAGE AT THE RECOMBINATION SITE
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DOI：
10.1016/0092-8674(81)90179-3
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发表时间：
1981-01-01
期刊：
影响因子：
64.5
通讯作者：
GRINDLEY, NDF
中科院分区：
文献类型：
--
作者：
REED, RR;GRINDLEY, NDF
解旋酶，转座因子γ的tnpR基因的产物。δ，介导在同一复制子上直接重复的该元件的2个拷贝之间的位点特异性重组[在大肠杆菌中]。解离酶作用的解离位点res位于tnpA和tnpR基因之间的顺反子间区域。在体外研究了这种位点特异性重组。在不存在Mg 2+的情况下，形成解离酶-res复合物，其含有已在res处切割的DNA分子。在该复合物中，解离酶可共价连接至切割的DNA的5 "末端，留下游离的3"-羟基。DNA切割是由同一底物分子上的2个res位点的相互作用刺激的，并且似乎是正常res位点重组中的中间步骤。在先前鉴定为包含回文序列处的交叉点的区域内切割DNA。图形 **。以生成2个碱基的3 "延伸。
Resolvase, the product of the tnpR gene of the transposable element .gamma..delta., mediates a site-specific recombination [in Escherichia coli] between 2 copies of the element directly repeated on the same replicon. The resolution site, res, at which resolvase acts lies in the intercistronic region between the tnpA and tnpR genes. This site-specific recombination was studied in vitro. In the absence of Mg2+, a resolvase-res complex is formed, which contains DNA molecules that have been cleaved at res. In this complex, resolvase may be covalently attached to the 5'' ends of the cleaved DNA, leaving free 3''-hydroxyl groups. DNA cleavage is stimulated by the interaction of 2 res sites on the same substrate molecule and appears to be an intermediate step in normal res site recombination. The DNA is cut within a region previously identified as containing the crossover point at the palindromic sequence .**GRAPHIC**. to generate 3'' extensions of 2 bases.