Polyphosphate accumulation in Escherichia coli in response to defects in DNA metabolism.
Polyphosphate accumulation in Escherichia coli in response to defects in DNA metabolism.
复制标题
大肠杆菌中聚磷酸盐的积累是对 DNA 代谢缺陷的反应。
DOI：
10.1128/jb.01138-09
复制
发表时间：
2009
影响因子：
3.2
通讯作者：
Kuzminov,Andrei
中科院分区：
文献类型：
--
作者：
Amado,Luciana;Kuzminov,Andrei
[~(32)P]正磷酸盐标记的大肠埃希氏菌的苯酚-氯仿抽提和碱性凝胶电泳显示，除了预期的染色体DNA外，还有两个非DNA物种，我们用生物化学和基因技术相结合的方法鉴定了它们是脂多糖和多聚磷酸盐。我们使用这个偶然发现的直接多聚磷酸盐检测方法来量化多聚磷酸盐水平。DNA代谢存在不同缺陷的共突变株。我们检测到在LigA、LIGA、RecBCD、Dutung和Ty突变体中聚磷酸盐积累增加。因此，聚磷酸盐的积累可能是普遍的DNA胁迫的一个指标。
Phenol-chloroform extraction of [32P]orthophosphate-labeledEscherichia colicells followed by alkaline gel electrophoresis revealed, besides the expected chromosomal DNA, two non-DNA species that we have identified as lipopolysaccharides and polyphosphates by using a combination of biochemical and genetic techniques. We used this serendipitously found straightforward protocol for direct polyphosphate detection to quantify polyphosphate levels inE. colimutants with diverse defects in the DNA metabolism. We detected increased polyphosphate accumulation in theligA,ligA recBCD,dut ung, andthyAmutants. Polyphosphate accumulation may thus be an indicator of general DNA stress.