Pseudoloma neurophilia n. g., n. sp., a new microsporidium from the central nervous system of the zebrafish (Danio rerio)
Pseudoloma neurophilia n. g., n. sp., a new microsporidium from the central nervous system of the zebrafish (Danio rerio)
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DOI：
10.1111/j.1550-7408.2001.tb00307.x
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发表时间：
2001-03-01
影响因子：
2.2
通讯作者：
Kent, ML
中科院分区：
文献类型：
--
作者：
Matthews, JL;Brown, AMV;Kent, ML
在俄勒冈州尤金的一个实验室群体中，在濒临死亡的斑马鱼的中枢神经系统中发现了一种不寻常的异种瘤形成微孢子虫。受感染的鱼通常是消瘦和昏昏欲睡的，组织学检查通常显示与感染有关的严重脊髓炎和肌炎。根据其结构、发育和小亚基核糖体DNA序列，它在鱼类微孢子虫中是独一无二的。孢子单核，卵圆形到梨形，后端有一个突出的液泡。孢子平均为5.4×2，7微米，极丝盘绕13-16圈。微孢子虫在鱼的脊髓和后脑产生异种瘤，异种瘤含有含孢子囊的多达16个孢子。孢子母细胞和孢子母细胞前阶段(可能是孢子体)偶尔会出现在散布在异种瘤中的小聚集体中。根据rDNA序列分析，它与其他鱼类微孢子虫属聚为“鱼孢子虫群”。斑马鱼微孢子虫的rDNA序列与鱼孢子虫的rDNA序列最相似，但仅有12.1%的相似性，因此该微孢子虫可被认为是一个不同的属和种，我们将其命名为神经嗜血假单胞菌。N.sp.
An unusual xenoma-forming microsporidium was discovered in the central nervous system of moribund zebrafish from a laboratory colony in Eugene, Oregon. Infected fish were often emaciated and lethargic, and histological examination commonly revealed severe myelitis and myositis associated with the infection. Based on its structure, development, and small subunit ribosomal DNA sequence it is unique among fish microsporidia. Spores are uninucleate, ovoid to pyriform, with a prominent posterior vacuole. Spores average 5.4 x 2,7 mum with 13-16 coils of the polar filament. The microsporidium produces xenomas within the spinal cord and hindbrain of fish, and xenomas contained sporophorous vesicles with up to 16 spores. Sporoblasts and presporoblast stages (probably sporonts) are found occasionally in small aggregates dispersed randomly throughout xenomas. It clustered in the "Ichthyosporidium group" along with other fish microsporidian genera based on rDNA sequence analysis. The rDNA sequence of the zebrafish microsporidium was most similar to that of Ichthyosporidium, but showed only 12.1% similarity and therefore this microsporidium can be considered a distinct genus and species, which we have named Pseudoloma neurophilia n. g.. n. sp.