Identification of cassava alternative splicing-related genes and functional characterization of MeSCL30 involvement in drought stress
Identification of cassava alternative splicing-related genes and functional characterization of MeSCL30 involvement in drought stress
复制标题
木薯选择性剪接相关基因的鉴定和 MeSCL30 参与干旱胁迫的功能表征。
DOI：
10.1016/j.plaphy.2021.01.016
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发表时间：
2021-01-21
影响因子：
6.5
通讯作者：
Wang, Zhen-Yu
中科院分区：
文献类型：
--
作者：
Weng, Xun;Zhou, Xiaoxia;Wang, Zhen-Yu
选择性剪接（alternative splicing，AS）是真核生物中一种重要的转录后调控策略，可以增加蛋白质组多样性，调节mRNA水平。多外显子基因可以选择性剪接以产生两个或更多个转录物，从而增加植物对外部胁迫条件的适应。然而，在热带地区重要的主食作物木薯中，AS相关蛋白的研究较少。在木薯基因组中共鉴定并命名了365个编码AS相关蛋白的基因，并系统研究了15个随机选择的基因在不同非生物胁迫条件下的转录和剪接变化。15个基因中有13个在组织中和不同的环境胁迫条件下发生AS。重要的是，AS相关蛋白的剪接模式在叶片或温度胁迫下发生了最大的变化，表明AS相关蛋白具有组织特异性的调控模式，可能参与了植物对温度胁迫的适应。我们发现MeSCL 30在拟南芥中的过表达通过维持活性氧（ROS）的稳态和增加干旱响应基因的表达来增强对干旱胁迫的耐受性。因此，本研究进一步完善了AS相关蛋白的编码基因，为调控AS相关基因以提高植物对环境胁迫的抗性提供了新的思路。
Alternative splicing (AS) is an important post-transcriptional regulation strategy that can increase the proteome diversity and regulate mRNA level in eukaryote. Multi-exon genes can be alternative spliced to generate two or more transcripts, thereby increasing the adaptation to the external stress conditions in planta. However, AS-related proteins were less explored in cassava which is an important staple crop in the tropical area. A total of 365 genes encoding AS-related proteins were identified and renamed in the cassava genome, and the transcriptional and splicing changes of 15 randomly selected genes were systematically investigated in the tissues under diverse abiotic stress conditions. 13 out of 15 genes undergo AS in the tissues and under diverse environmental stress condition. Importantly, the greatest changes of splicing patterns were found in the leaf or in response to temperature stress, indicating that AS-related proteins had their tissue-specific regulation patterns and might be participated in the plant adaptation to temperature stress. We then found that overexpression of MeSCL30 in Arabidopsis enhanced the tolerance to drought stress through maintaining reactive oxygen species (ROS) homeostasis and increasing the expression of drought-responsive genes. Therefore, these findings refined the AS-related protein-coding genes and provided novel insights for manipulation of AS-related genes in order to enhance the resistance to environmental stress in plant.