Overexpression of SUGT1 in human colorectal cancer and its clinicopathological significance
Overexpression of SUGT1 in human colorectal cancer and its clinicopathological significance
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DOI：
10.3892/ijo_00000531
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发表时间：
2010-03-01
影响因子：
5.2
通讯作者：
Mori, Masaki
中科院分区：
文献类型：
--
作者：
Iwatsuki, Masaaki;Mimori, Koshi;Mori, Masaki
由于最近的技术创新使得阐明结直肠癌（CRC）进展过程中基因组改变与基因异常表达之间的一致性关系成为可能，我们旨在鉴定结直肠癌中相关位点上具有基因组扩增的新过表达基因。利用激光显微解剖（LMD）后的cDNA微阵列和基于阵列的比较基因组杂交（CGH）分析，发现了132例日本结直肠癌的候选基因。我们重点研究了SUGT1，它与细胞周期中期着丝点蛋白的组装有关，在遗传改变和表达之间存在显著关联。我们对98例结直肠癌患者的SUGT1 mRNA表达进行了评估，以确定SUGT1表达的临床病理意义。肿瘤组织标本中SUGT1 mRNA的平均表达水平显著高于非肿瘤组织。SUGT1高表达组的特点是复发频率明显高于低表达组，预后明显较差。结直肠癌患者预后不良与SUGT1过表达有显著相关性，且该基因座的基因组扩增一致。SUGT1基因的扩增可能直接促进了该基因的转录，从而导致CRC患者的预后恶化。
As recent technological innovations make it possible to clarify the concordant relationship between genomic alterations and aberrant gene expression during the progression of colorectal cancer (CRC), we aimed at identifying new overexpressing genes with genomic amplification on the responsible loci in CRC. The candidate gene was found using cDNA microarray and array-based comparative genomic hybridization (CGH) analysis after laser microdissection (LMD) in 132 Japanese CRC. We focused on SUGT1, which is associated with the assembling of kinetochore proteins at the metaphase of the cell cycle, with significant association between genetic alterations and expression. SUGT1 mRNA expression was evaluated in 98 CRC cases to determine the clinicopathological significance of SUGT1 expression. The mean level of SUGT1 mRNA expression in tumor tissue specimens was significantly higher than in non-tumor tissue. The high SUGT1 expression group was characterized by a significantly elevated frequency of recurrence and a significantly poorer prognosis than the low expression group. There was a significant association between poor prognosis of CRC cases and the overexpression of SUGT1 with genomic amplification of the loci concordantly. The amplification of SUGT1 might give rise to promote the transcription of the gene directly subsequent to the progression of CRC cases with worsening prognosis.