Tissue Engineering the Cornea: The Evolution of RAFT.
Tissue Engineering the Cornea: The Evolution of RAFT.
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DOI：
10.3390/jfb6010050
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发表时间：
2015-01-22
影响因子：
4.8
通讯作者：
Daniels JT
中科院分区：
文献类型：
--
作者：
Levis HJ;Kureshi AK;Massie I;Morgan L;Vernon AJ;Daniels JT
角膜失明影响着全世界超过 1000 万人，当前的治疗策略通常涉及用健康组织替换有缺陷的层。由于全球范围内供体角膜短缺和缺乏合适的生物支架，最近的研究重点是开发组织工程技术以创造替代疗法。这篇综述将详细介绍我们如何将胶原蛋白塑料压缩的简单工程技术改进为我们现在称为 3D 组织真实架构 (RAFT) 的过程。 RAFT 生产流程已标准化，并已采取措施考虑良好生产规范合规性。这一过程的发展使我们能够创建适合移植的仿生上皮和内皮组织等同物，并且是体外研究细胞间相互作用的理想选择。
Corneal blindness affects over 10 million people worldwide and current treatment strategies often involve replacement of the defective layer with healthy tissue. Due to a worldwide donor cornea shortage and the absence of suitable biological scaffolds, recent research has focused on the development of tissue engineering techniques to create alternative therapies. This review will detail how we have refined the simple engineering technique of plastic compression of collagen to a process we now call Real Architecture for 3D Tissues (RAFT). The RAFT production process has been standardised, and steps have been taken to consider Good Manufacturing Practice compliance. The evolution of this process has allowed us to create biomimetic epithelial and endothelial tissue equivalents suitable for transplantation and ideal for studying cell-cell interactions in vitro.