Evaluation of direct analysis in real time ionization-mass spectrometry (DART-MS) in fish metabolomics aimed to assess the response to dietary supplementation
Evaluation of direct analysis in real time ionization-mass spectrometry (DART-MS) in fish metabolomics aimed to assess the response to dietary supplementation
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DOI：
10.1016/j.talanta.2013.04.025
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发表时间：
2013-10-15
期刊：
影响因子：
6.1
通讯作者：
Hajslova, Jana
中科院分区：
文献类型：
--
作者：
Cajka, Tomas;Danhelova, Hana;Hajslova, Jana
采用真实的时间直接分析（DART）离子源与中高分辨率/高精度质量飞行时间质谱（TOFMS）联用的环境质谱（Ambient MS）技术，研究了添加小黑麦对鲤鱼肌肉代谢物组成的影响。首先，样品提取和DART TOFMS仪器条件进行了优化，以获得最广泛的代表性的可电离化合物发生在从鲤鱼肌肉提取物。为此，同时（所有在一个）提取程序开发采用水和环己烷混合物作为提取溶剂。在这些条件下，极性和非极性代谢产物均在单个浸提步骤中分离。其次，获得了大量鲤鱼肌肉提取物的代谢组学指纹图谱（质谱）。最后，采用主成分分析（PCA）和正交偏最小二乘判别分析（OPLS-DA）对试验数据进行了统计分析，结果表明，该方法可以区分鲤鱼肌肉对饲料添加（添加和不添加谷物）的反应。根据极性和非极性浸提液指纹图谱的评估，获得了正确的分类。目前的研究表明，DART TOFMS代谢组学指纹图谱是一种快速而强大的分析策略，通过记录环境MS条件下可电离组分的代谢组学指纹图谱，可以根据饲养历史区分鲤鱼肌肉。（C）2013 Elsevier B. V.保留所有权利。
Ambient mass spectrometry employing a direct analysis in real time (DART) ion source coupled to a medium high-resolution/accurate mass time-of-flight mass spectrometer (TOFMS) was used as a rapid tool for metabolomic fingerprinting to study the effects of supplemental feeding with cereals (triticale) on the composition of muscle metabolites of common carp (Cyprinus carpio L.).First, the sample extraction and DART TOFMS instrumental conditions were optimized to obtain the broadest possible representation of ionizable compounds occurring in the extracts obtained from common carp muscle. To this end, a simultaneous (all-in-one) extraction procedure was developed employing water and cyclohexane mixture as the extraction solvents. Under these conditions both polar as well as non-polar metabolites were isolated within a single extraction step. Next, the metabolomic fingerprints (mass spectra) of a large set of common carp muscle extracts were acquired. Finally, the experimental data were statistically evaluated using principal component analysis (PCA) and orthogonal partial least squares discriminant analysis (OPLS-DA).Using this approach, differentiation of common carp muscle in response to dietary supplementation (feeding with and without cereals) was feasible. Correct classification was obtained based on the assessment of polar and as well as non-polar extracts fingerprints. The current study showed that DART TOFMS metabolomic fingerprinting represents a rapid and powerful analytical strategy enabling differentiation of common carp muscles according to feeding history by recording metabolomic fingerprints of ionizable components under the conditions of ambient MS. (C) 2013 Elsevier B.V. All rights reserved.