Tumorsphere assay provides more accurate prediction of in vivo responses to chemotherapeutics.
Tumorsphere assay provides more accurate prediction of in vivo responses to chemotherapeutics.
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DOI：
10.1007/s10529-013-1393-1
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发表时间：
2014-03
影响因子：
2.7
通讯作者：
Alexander, Caroline M.
中科院分区：
文献类型：
--
作者：
Kim, Soyoung;Alexander, Caroline M.
关键词：
Although the sphere culture system has been widely used in stem cell biology, its application for drug screening is limited due to lack of standardized, rapid analytical tools. To optimize sphere cultures for in vitro screening of drugs, we evaluated the properties of primary tumor cells growing as tumorspheres and compared their chemosensitivity to those of cells growing in monolayer. Most cells in tumorsphere cultures were quiescent whereas cells in monolayer culture had a high mitotic index. Moreover, doxorubicin showed better cytotoxicity than paclitaxel in the sphere cultures, but their efficacy was reversed in the monolayer cultures. Importantly, the response of cytotoxic outcomes for suspension cultures matched the in vivo response better than monolayer cultures, providing support for the use of short term suspension cultures of primary cells as a model for drug testing.
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影响因子：
10.5
作者：
Dontu, G;Abdallah, WM;Wicha, MS
通讯作者：
Wicha, MS
DOI：
10.1083/jcb.139.3.575
发表时间：
1997-11-03
期刊：
The Journal of cell biology
影响因子：
--
作者：
Schwartz MA
通讯作者：
Schwartz MA
影响因子：
64.8
作者：
Greaves, Mel;Maley, Carlo C.
通讯作者：
Maley, Carlo C.
影响因子：
8.8
作者：
Gamucci T;D'Ottavio AM;Magnolfi E;Barduagni M;Vaccaro A;Sperduti I;Moscetti L;Belli F;Meliffi L
通讯作者：
Meliffi L
DOI：
10.1186/bcr2106
发表时间：
2008
期刊：
Breast cancer research : BCR
影响因子：
--
作者：
Grimshaw MJ;Cooper L;Papazisis K;Coleman JA;Bohnenkamp HR;Chiapero-Stanke L;Taylor-Papadimitriou J;Burchell JM
通讯作者：
Burchell JM