Effect of Diclazuril on the Bursa of Fabricius Morphology and SIgA Expression in Chickens Infected with Eimeria tenella.
Effect of Diclazuril on the Bursa of Fabricius Morphology and SIgA Expression in Chickens Infected with Eimeria tenella.
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地克珠利对柔嫩艾美耳球虫感染鸡法氏囊形态及SIgA表达的影响
DOI：
10.3347/kjp.2015.53.6.675
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发表时间：
2015-12
期刊：
影响因子：
--
通讯作者：
Wang HW
中科院分区：
文献类型：
--
作者：
Zhou BH;Liu LL;Liu J;Yuan FW;Tian EJ;Wang HW
研究了地克珠利对感染柔嫩艾美耳球虫（Eimeria tenella）雏鸡腔上囊（BF）结构和分泌伊加（SIgA）表达的影响。苏木精-伊红染色观察BF形态，透射电镜观察BF超微结构变化。E. tenella感染引起BF细胞体积减小，排列不规则，细胞间隙增大。地克珠利治疗减轻了与E. tenella感染免疫组化法检测BF中SIgA的表达。SIgA表达在E.与正常对照组比较。地克珠利治疗后，皮质SIgA表达相对减少，与感染组和未治疗组相比，SIgA表达水平显著降低46.7%（P<0.01）。最后，E.鸡柔嫩杆菌感染可引起BF形态结构的明显改变，并刺激BF中SIgA的表达。地克珠利处理有效地减轻了形态学变化。该结果证明了在球虫病控制中开发免疫策略的方法。
The effects of diclazuril on the bursa of Fabricius (BF) structure and secretory IgA (SIgA) expression in chickens infected with Eimeria tenella were examined. The morphology of the BF was observed by hematoxylin and eosin staining, while ultrastructural changes were monitored by transmission electron microscopy. E. tenella infection caused the BF cell volumes to decrease, irregularly arranged, as well as, enlargement of the intercellular space. Diclazuril treatment alleviated the physical signs of damages associated with E. tenella infection. The SIgA expression in BF was analyzed by immunohistochemistry technique. The SIgA expression increased significantly by 350.4% (P<0.01) after E. tenella infection compared to the normal control group. With the treatment of diclazuril, the SIgA was relatively fewer in the cortex, and the expression level was significantly decreased by 46.7% (P<0.01) compared with the infected and untreated group. In conclusion, E. tenella infection in chickens induced obvious harmful changes in BF morphological structure and stimulated the expression of SIgA in the BF. Diclazuril treatment effectively alleviated the morphological changes. This result demonstrates a method to develop an immunological strategy in coccidiosis control.