Bactericidal Effect of Er:YAG Laser-Activated Sodium Hypochlorite Irrigation Against Biofilms of Enterococcus faecalis Isolate from Canal of Root-Filled Teeth with Periapical Lesions
Bactericidal Effect of Er:YAG Laser-Activated Sodium Hypochlorite Irrigation Against Biofilms of Enterococcus faecalis Isolate from Canal of Root-Filled Teeth with Periapical Lesions
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Er:YAG 激光激活次氯酸钠冲洗对根尖周病变根充牙根管中粪肠球菌生物膜的杀菌作用。
DOI：
10.1089/pho.2017.4293
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发表时间：
2017-07-01
影响因子：
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通讯作者：
Tian, Yu
中科院分区：
文献类型：
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作者：
Cheng, Xiaogang;Xiang, Doudou;Tian, Yu
目的：本研究旨在评价Er：YAG激光激活次氯酸钠冲洗液（Er：YAG + NaOCl）对粪肠球菌临床分离株生物膜的杀菌效果。背景资料：已有文献报道Er：YAG + NaOCl对实验室适应性大肠杆菌有有效的杀菌作用。faecalis菌株，而没有研究报道其对临床分离株的影响。方法：18株E.从39个根尖周病变的根充牙中分离粪菌，并使用结晶紫染色法评价其生物膜形成能力。用40#/0.04K3器械预备离体根管，用E.粪肠球菌分离株表现出最强的生物膜形成能力4周。感染根管分别用生理盐水（NS）或NaOCl冲洗，超声激活冲洗US + NS和US + NaOCl，Er：YAG激光激活冲洗Er：YAG + NS和Er：YAG + NaOCl。用扫描电镜（SEM）观察根管情况。使用细胞计数法评价细菌减少。结果：扫描电镜观察显示，细菌培养4周后，根管壁形成生物膜样结构。Er：YAG + NaOCl可完全去除E.粪菌生物膜从根管壁脱落，使其成为治疗组中最清洁和最光滑的表面。各治疗组细菌减少率依次为Er：YAG + NaOCl（98.8%）、US + NaOCl（98.6%）> NaOCl（94.0%）> Er：YAG + NS（91.9%）> US + NS（78.1%）> NS（51.1%）（p
Objective: This study was to evaluate the bactericidal effect of Er:YAG laser-activated sodium hypochlorite irrigation (Er:YAG + NaOCl) on biofilms of Enterococcus faecalis clinical isolate. Background data: It was reported that Er:YAG + NaOCl had effective bactericidal effect on laboratory-adapted E. faecalis strain, while no study has reported its effect on the clinical isolate. Methods: Eighteen E. faecalis strains were isolated from 39 root-filled teeth with periapical lesions, and their biofilm formation abilities were evaluated using the crystal violet staining method. Extracted human root canals were prepared to a 40#/.04 K3 instrument and contaminated with the E. faecalis isolate that presented the strongest biofilm formation ability for 4 weeks. The infected canals then received treatments of syringe irrigation with normal saline (NS) or NaOCl, ultrasonic activated irrigations US + NS and US + NaOCl, and Er:YAG laser-activated irrigations Er:YAG + NS and Er:YAG + NaOCl. The root canals were examined using scanning electron microscopy (SEM). The bacterial reductions were evaluated using the cell count method. Results: SEM results showed that biofilm-like structures formed on the root canal walls after 4-week bacterial incubation. Er:YAG + NaOCl completely removed the E. faecalis biofilm from the root canal wall and made it the cleanest and most smooth surface among the treatment groups. Bacterial reductions in the treatment groups were presented in a descending order of Er:YAG + NaOCl (98.8%), US + NaOCl (98.6%) > NaOCl (94.0%) > Er:YAG + NS (91.9%) > US + NS (78.1%) > NS (51.1%) (p