Improvement of hydroxyvalerate fraction in poly(beta-hydroxybutyrate-co-beta-hydroxyvalerate) by a mutant strain of Alcaligenes eutrophus
Improvement of hydroxyvalerate fraction in poly(beta-hydroxybutyrate-co-beta-hydroxyvalerate) by a mutant strain of Alcaligenes eutrophus
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DOI：
10.1016/0922-338x(96)82217-3
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发表时间：
1996-01-01
期刊：
影响因子：
--
通讯作者：
Park, YH
中科院分区：
文献类型：
--
作者：
Lee, IY;Kim, GJ;Park, YH
为了提高产碱菌产poly(beta-hydroxybutyrate-co-beta-hydroxyvalerate)[P(HB-co-HV)]的菌株活力，通过MNNG诱变获得了一株突变株BK23。与亲本菌株NCIMB 11599相比，该菌株的羟基戊酸酯(HV)组分和丙酸产量都有显著提高。在补料分批培养中，突变株的P(HB-co-HV)产量为47g/L，HV组分为22摩尔%，而亲本菌株的产量仅为10摩尔%。
In an effort to improve the strain activity of Alcaligenes eutrophus to produce poly(beta-hydroxybutyrate-co-beta-hydroxyvalerate) [P(HB-co-HV)], a mutant strain BK23 was obtained by MNNG mutagenesis. With the strain, the fraction of hydroxyvalerate (HV) and its yield from propionic acid were found to be significantly improved compared to those of the parent strain, A. eutrophus NCIMB 11599. In a fed-batch culture, the mutant strain produced 47 g/l of P(HB-co-HV) with an HV fraction of 22 mol%, while the parent strain could yield only the much lower fraction of 10 mol%.