Ischemic preconditioning protects against spinal cord ischemia-reperfusion injury in rabbits by attenuating blood spinal cord barrier disruption.
Ischemic preconditioning protects against spinal cord ischemia-reperfusion injury in rabbits by attenuating blood spinal cord barrier disruption.
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DOI：
10.3390/ijms140510343
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发表时间：
2013-05-17
影响因子：
5.6
通讯作者：
Ma H
中科院分区：
文献类型：
--
作者：
Fang B;Li XM;Sun XJ;Bao NR;Ren XY;Lv HW;Ma H
缺血预处理对脊髓缺血再灌注损伤具有保护作用，但其机制尚不完全清楚。为了研究这一点，日本白色家兔接受了I-R（30分钟主动脉闭塞，然后再灌注）、缺血预处理（3个循环的5分钟主动脉闭塞加5分钟再灌注），然后进行I-R或假手术。在再灌注后4和24 h，使用Tarlov评分评估神经功能，使用Evan's Blue外渗测量血脊髓屏障通透性，使用湿-干法评估脊髓水肿，以及脊髓闭合小带-1（ZO-1）、基质金属蛋白酶-9（MMP-9）、Western blot和实时荧光定量聚合酶链反应检测肿瘤坏死因子-α（TNF-α）。还使用免疫荧光评估ZO-1。脊髓I-R损伤降低神经功能评分，缺血预处理可改善这种效应。缺血预处理抑制缺血再灌注引起的血脊髓屏障通透性和含水量的增加，增加ZO-1 mRNA和蛋白表达，降低MMP-9和TNF-α mRNA和蛋白表达。提示缺血预处理通过保护紧密连接蛋白ZO-1和降低MMP-9和TNF-α的表达，减轻了脊髓缺血再灌注损伤后血脊髓屏障通透性的增加。
Ischemic preconditioning has been reported to protect against spinal cord ischemia-reperfusion (I-R) injury, but the underlying mechanisms are not fully understood. To investigate this, Japanese white rabbits underwent I-R (30 min aortic occlusion followed by reperfusion), ischemic preconditioning (three cycles of 5 min aortic occlusion plus 5 min reperfusion) followed by I-R, or sham surgery. At 4 and 24 h following reperfusion, neurological function was assessed using Tarlov scores, blood spinal cord barrier permeability was measured by Evan’s Blue extravasation, spinal cord edema was evaluated using the wet-dry method, and spinal cord expression of zonula occluden-1 (ZO-1), matrix metalloproteinase-9 (MMP-9), and tumor necrosis factor-α (TNF-α) were measured by Western blot and a real-time polymerase chain reaction. ZO-1 was also assessed using immunofluorescence. Spinal cord I-R injury reduced neurologic scores, and ischemic preconditioning treatment ameliorated this effect. Ischemic preconditioning inhibited I-R-induced increases in blood spinal cord barrier permeability and water content, increased ZO-1 mRNA and protein expression, and reduced MMP-9 and TNF-α mRNA and protein expression. These findings suggest that ischemic preconditioning attenuates the increase in blood spinal cord barrier permeability due to spinal cord I-R injury by preservation of tight junction protein ZO-1 and reducing MMP-9 and TNF-α expression.
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影响因子：
14.5
作者：
Lee, Jee Y.;Kim, Hwang S.;Yune, Tae Y.
通讯作者：
Yune, Tae Y.
促红细胞生成素对缺血再灌注大鼠血脑屏障紧密连接的影响
影响因子：
3.1
作者：
Liu, Kun;Sun, Tao;Xue, Yi-xue
通讯作者：
Xue, Yi-xue
DOI：
10.1016/j.ejvs.2011.12.028
发表时间：
2012-04-01
影响因子：
5.7
作者：
Kyrou, I. E.;Papakostas, J. C.;Matsagkas, M. I.
通讯作者：
Matsagkas, M. I.
影响因子：
4.6
作者：
Shi, Enyi;Kazui, Teruhisa;Bashar, Abul Hasan Muhammad
通讯作者：
Bashar, Abul Hasan Muhammad
影响因子：
5.7
作者：
Saito, Takeshi;Tsuchida, Masanori;Hayashi, Jun-ichi
通讯作者：
Hayashi, Jun-ichi