Cleavage of oligodeoxyribonucleotides from controlled-pore glass supports and their rapid deprotection by gaseous amines
Cleavage of oligodeoxyribonucleotides from controlled-pore glass supports and their rapid deprotection by gaseous amines
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DOI：
10.1093/nar/24.15.3115
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发表时间：
1996-08-01
影响因子：
14.9
通讯作者：
Beaucage, SL
中科院分区：
文献类型：
--
作者：
Boal, JH;Wilk, A;Beaucage, SL
开发了一种新的寡脱氧核苷酸脱保护方法。在压力下使用气态胺如氨或甲胺以实现温和和快速的脱保护条件。例如，具有用于保护胞嘧啶、腺嘌呤和鸟嘌呤的环外氨基官能团的（叔丁基）苯氧基乙酰基的寡脱氧核糖核苷酸从可控孔玻璃载体释放，并在气相条件下，在室温下，分别在35或2分钟内通过氨或甲胺完全脱保护。
A novel method for the deprotection of oligodeoxyribonucleotides has been developed. Gaseous amines such as ammonia or methylamine were employed under pressure to achieve mild and rapid deprotection conditions. For example, oligodeoxyribonucleotides having a (tent-butyl)phenoxyacetyl group for the protection of the exocyclic amino function of cytosine, adenine and guanine were released from controlled-pore glass supports and fully deprotected by ammonia or methylamine under gas phase conditions, at room temperature, within 35 or 2 min respectively.