Stimulation of heme accumulation and erythroid colony formation in cultures of chick bone marrow cells by chicken plasma.
Stimulation of heme accumulation and erythroid colony formation in cultures of chick bone marrow cells by chicken plasma.
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通过鸡等离子体刺激血红素积累和红细胞菌落形成。
DOI：
10.1083/jcb.76.1.184
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发表时间：
1978-01
影响因子：
7.8
通讯作者：
Ingram, V M
中科院分区：
文献类型：
--
作者：
Coll, J;Ingram, V M
本文报道了一种用于鸡骨髓红系细胞生长的高平板效率纤维蛋白凝块培养系统。在不存在成年鸡血浆的情况下生长的红系菌落（自发菌落）为联苯胺阴性或弱联苯胺阳性。在鸡血浆存在下生长的菌落为90%强联苯胺阳性，比自发菌落多40%。贫血鸡的血浆在诱导血红素积累（血红素刺激活性）和增加红系细胞集落（集落刺激活性）方面比对照血浆更有效。通过将48小时培养物的自发菌落暴露于鸡血浆6-10小时，将其转化为联苯胺阳性菌落。
A fibrin clot culture system with high plating efficiency is described for the growth of erythroid cells from chick bone marrow. Erythroid colonies grown in the absence of adult chicken plasma (spontaneous colonies) were either benzidine-negative or weakly benzidine-positive. Colonies grown in the presence of chicken plasma were 90% strongly benzidine-positive and 40% more abundant than spontaneous colonies. Plasma from anemic chickens was more effective than control plasma in inducing heme accumulation (heme-stimulating activity) and in increasing the number of erythroid colonies (colony-stimulating activity). Spontaneous colonies from 48-h cultures were transformed into benzidine-positive colonies by exposing them for 6-10 h to chicken plasma.