Regenerant Arabidopsis lineages display a distinct genome-wide spectrum of mutations conferring variant phenotypes.
Regenerant Arabidopsis lineages display a distinct genome-wide spectrum of mutations conferring variant phenotypes.
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DOI：
10.1016/j.cub.2011.07.002
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发表时间：
2011-08-23
期刊：
影响因子：
--
通讯作者：
Harberd NP
中科院分区：
文献类型：
--
作者：
Jiang C;Mithani A;Gan X;Belfield EJ;Klingler JP;Zhu JK;Ragoussis J;Mott R;Harberd NP
多细胞生物可以从全能分化的体细胞或核创始人再生。从克隆相关的同基因创始人再生的生物体可能是先验的表型不变。然而，克隆再生动物表现出由基因表达的缺陷性表观遗传重编程引起的变异表型，克隆再生植物表现出知之甚少的可遗传表型（“体细胞克隆”）变异。在这里，我们表明，再生拟南芥谱系的体细胞克隆变异与DNA序列突变率的全基因组海拔。我们还表明，再生突变包括一个独特的分子谱的碱基取代，插入和缺失，可能导致DNA修复保真度下降。最后，我们表明，虽然再生碱基替换是一个可能的主要遗传原因的体细胞克隆变异的再生拟南芥谱系，转座子运动是不太可能作出重大贡献的变化。我们的结论是，再生植物的表型变异，不像再生动物，基本上是由于DNA序列突变。再生拟南芥谱系显示可遗传的表型变异再生拟南芥谱系显示升高的全基因组DNA序列突变再生DNA序列突变包含不同的分子谱再生碱基取代突变赋予可遗传的表型变异
Multicellular organisms can be regenerated from totipotent differentiated somatic cell or nuclear founders. Organisms regenerated from clonally related isogenic founders might a priori have been expected to be phenotypically invariant. However, clonal regenerant animals display variant phenotypes caused by defective epigenetic reprogramming of gene expression, and clonal regenerant plants exhibit poorly understood heritable phenotypic (“somaclonal”) variation. Here we show that somaclonal variation in regenerant Arabidopsis lineages is associated with genome-wide elevation in DNA sequence mutation rate. We also show that regenerant mutations comprise a distinctive molecular spectrum of base substitutions, insertions, and deletions that probably results from decreased DNA repair fidelity. Finally, we show that while regenerant base substitutions are a likely major genetic cause of the somaclonal variation of regenerant Arabidopsis lineages, transposon movement is unlikely to contribute substantially to that variation. We conclude that the phenotypic variation of regenerant plants, unlike that of regenerant animals, is substantially due to DNA sequence mutation. ► Regenerant Arabidopsis lineages display heritable phenotypic variation ► Regenerant Arabidopsis lineages display elevated genome-wide DNA sequence mutation ► Regenerant DNA sequence mutations comprise a distinct molecular spectrum ► Regenerant base substitution mutations confer heritable phenotypic variation
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影响因子：
11.4
作者：
Lynch, Michael
通讯作者：
Lynch, Michael
影响因子：
7.2
作者：
Dieterle, M;Thomann, A;Genschik, P
通讯作者：
Genschik, P
DOI：
10.1073/pnas.93.15.7783
发表时间：
1996-07-23
影响因子：
11.1
作者：
Hirochika, H;Sugimoto, K;Kanda, M
通讯作者：
Kanda, M
影响因子：
2.3
作者：
HEINZ, DJ;MEE, GWP
通讯作者：
MEE, GWP
影响因子：
56.9
作者：
Humpherys, D;Eggan, K;Jaenisch, R
通讯作者：
Jaenisch, R