Molecular cloning and functional expression of a Drosophila corazonin receptor
Molecular cloning and functional expression of a Drosophila corazonin receptor
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DOI：
10.1016/s0006-291x(02)02398-7
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发表时间：
2002-10-18
影响因子：
3.1
通讯作者：
Grimmelikhuijzen, CJP
中科院分区：
文献类型：
--
作者：
Cazzamali, G;Saxild, NPE;Grimmelikhuijzen, CJP
昆虫脂肪运动激素（AKH）是一个神经肽家族，在昆虫的飞行等需要能量的活动中从脂肪体中动员脂肪和糖。我们先前已经从果蝇Drosophila melanogaster和家蚕Bombyx mori鉴定了第一种昆虫AKH受体（Staubli et al.，PNAS 2002，99：3446-3451）。在这里，我们克隆了果蝇G蛋白偶联受体的cDNA，该受体与第一个果蝇AKH受体在氨基酸序列和基因结构方面都密切相关。随后，我们表达了这种孤儿受体在中国仓鼠卵巢细胞，并确定果蝇corazonin作为受体的内源性配体。Corazonin在某些昆虫中增加心跳，但它在果蝇中的功能尚不清楚。这些结果是有趣的，因为不仅是果蝇AKH和corazonin受体的结构和进化相关，但也是他们的preprohormones，这表明配体和受体的共同进化。果蝇corazonin受体在胚胎、幼虫、蛹和成虫中表达。此外，在疟疾蚊子冈比亚按蚊的基因组数据库中可以发现一种结构上与果蝇corazonin受体非常相似的受体。(C)2002 Elsevier Science（美国）。All rights reserved.
The insect adipokinetic hormones (AKHs) constitute a large family of neuropeptides that mobilize lipids and sugar from the insect fat body during energy-requiring activities such as flight. We have previously identified the first insect AKH receptors from the fruitfly Drosophila melanogaster and the silkworm Bombyx mori (Staubli et al., PNAS 2002, 99: 3446-3451). Here, we have cloned the cDNA of a Drosophila G protein-coupled receptor that was closely related to the first Drosophila AKH receptor both with respect to amino-acid sequence and gene structure. We have subsequently expressed this orphan receptor in Chinese hamster ovary cells and identified Drosophila corazonin as the endogenous ligand for the receptor. Corazonin increases heart beat in some insects, but its function in Drosophila is unknown. These results are intriguing, because not only are the Drosophila AKH and corazonin receptors structurally and evolutionarily related, but also are their preprohormones, which suggests a co-evolution of ligands and receptors. The Drosophila corazonin receptor is expressed in embryos, larvae, pupae, and adult flies. Furthermore, a receptor that is structurally very similar to the Drosophila corazonin receptor can be found in the genomic database from the malaria mosquito Anopheles gambiae. (C) 2002 Elsevier Science (USA). All rights reserved.