The influence of pH on cystine and dibasic amino acid transport by rat renal brushborder membrane vesicles.
The influence of pH on cystine and dibasic amino acid transport by rat renal brushborder membrane vesicles.
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pH对大鼠肾刷状缘膜囊泡胱氨酸和二元氨基酸转运的影响。
DOI：
10.1016/0304-4165(91)90039-j
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发表时间：
1991
期刊：
影响因子：
--
通讯作者：
Segal,S
中科院分区：
文献类型：
--
作者：
Reynolds,RA;Mahoney,SG;McNamara,PD;Segal,S
The uptake of cystine and lysine by rat renal brushborder membrane vesicles was examined at various intravesicular and extravesicular hydrogen ion concentrations to discern whether ionic species are determinants of specificity for the shared transport system and whether hydrogen ion gradients play a role in determining uptake values. When intravesicular and extravesicular pH are identical, the highest uptake of cystine occurred at pH 7.4, with lesser uptake at pH 6.0 and 8.3. Since cystine is electroneutral at pH 6.0 and 90% anionic at pH 8.3, it appears that neither form of the amino acid is a preferred species for transport. A similar relationship between pH and uptake occurs for lysine, which is cationic at pH below 8.5. This suggests that pH affects the functioning of the membrane carrier system independent of ionic species of the substrate. There is no apparent relationship of cystine uptake to hydrogen ion gradients across the membrane. Over the range of extravesicular pH studied, optimal cystine uptake occurred whenever the intravesicular pH was 7.4. Competitive interactions between unlabeled amino acids and labeled cystine were not effected by the extravesicular pH and, therefore, did not seem determined by the ionic species of cystine.
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DOI：
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发表时间：
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期刊：
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影响因子：
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作者：
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