Localization of double bonds in triacylglycerols using high-performance liquid chromatography/atmospheric pressure chemical ionization ion-trap mass spectrometry
Localization of double bonds in triacylglycerols using high-performance liquid chromatography/atmospheric pressure chemical ionization ion-trap mass spectrometry
复制标题
DOI：
10.1007/s00216-015-8537-1
复制
发表时间：
2015-07-01
影响因子：
4.3
通讯作者：
Cvacka, Josef
中科院分区：
文献类型：
--
作者：
Hakova, Eva;Vrkoslav, Vladimir;Cvacka, Josef
建立了一种高效液相色谱-串联质谱常压化学电离法（APCI）定位三酰基甘油双键的方法。该技术是基于碰撞诱导解离或脉冲Q碰撞诱导解离在APCI源中乙腈存在下形成的C3H5N+aEuro cent加合物（[M + 55](+aEuro cent)）。光谱的研究使用了从商业来源获得的一系列标准，并通过随机化制备。裂解谱可以确定(i)分子中碳和双键的总数，（ii）酰基中的碳和双键的数目，（iii）甘油主链上sn-2位置的酰基，（iv）酰基中双键的位置。双键的位置是根据C-C键乙烯基与原双键断裂形成的两种片段（α和ω离子）来确定的。酰基的组成及其在甘油上的位置是根据[M + 55]（+ 1欧分）前体消除脂肪酸形成的离子的质量和强度确定的。将该方法应用于橄榄油和鹅皮脂中甘油三酯的分析。
A method for localizing double bonds in triacylglycerols using high-performance liquid chromatography-tandem mass spectrometry with atmospheric pressure chemical ionization (APCI) was developed. The technique was based on collision-induced dissociation or pulsed Q collision-induced dissociation of the C3H5N+aEuro cent adducts ([M + 55](+aEuro cent)) formed in the presence of acetonitrile in the APCI source. The spectra were investigated using a large series of standards obtained from commercial sources and prepared by randomization. The fragmentation spectra made it possible to determine (i) the total number of carbons and double bonds in the molecule, (ii) the number of carbons and double bonds in acyls, (iii) the acyl in the sn-2 position on the glycerol backbone, and (iv) the double-bond positions in acyls. The double-bond positions were determined based on two types of fragments (alpha and omega ions) formed by cleavages of C-C bonds vinylic to the original double bond. The composition of the acyls and their positions on glycerol were established from the masses and intensities of the ions formed by the elimination of fatty acids from the [M + 55](+aEuro cent) precursor. The method was applied for the analysis of triacylglycerols in olive oil and vernix caseosa.