Development of palm-sized differential plasmon resonance meter based on concept of Sprode
Development of palm-sized differential plasmon resonance meter based on concept of Sprode
复制标题
DOI：
10.1016/j.snb.2004.12.103
复制
发表时间：
2005-07-22
影响因子：
8.4
通讯作者：
Oinuma, T
中科院分区：
文献类型：
--
作者：
Hemmi, A;Imato, T;Oinuma, T
针对内分泌干扰物现场分析中表面等离子体共振（SPR）仪器的小型化问题，研制了一种由单光源、棱镜、分镜和线阵CCD组成的手掌大小的差动式SPR仪器。测量仪的尺寸和重量分别为160 cm（W）x 10 cm（H）x 6 cm（D）和770 g。由Sprode与样品和参考传感器单元组成的SPR仪被发现对于补偿传感器信号的漂移和波动以及同时无时滞的温度变化是非常有效的。有效性是由于参考信号从样本信号的减法函数。测量仪的基本性能是在4300 s内SPR测量中的0.001度内的漂移和在10度入射LED光束下的0.0004度内的角度稳定性。此外，SPR仪应用于免疫分析的2，4-二氯苯酚（2，4-DCP），使用固相萃取技术。用该仪器测得的2，4-DCP浓度与SPR信号之间有良好的线性关系。用研制的仪器可以测定10 ppb的2，4-DCP。一个新的概念，分割镜和Sprode被认为是有用的小型化的SPR仪器，旨在现场分析。(c)2005 Elsevier B. V.保留所有权利。
A palm-sized differential surface plasmon resonance (SPR) meter, which consisted of a single light source, a prism, a divided mirror, and a single linear CCD element, was developed aiming at the miniaturization of an SPR instrument for use in field analysis of endocrine disrupting chemicals. The size and weight of the meter were 160 cm (W) x 10 cm (H) x 6 cm (D) and 770 g, respectively. The SPR meter composed of an Sprode with the sample and reference sensor-cells was found to be very effective for compensating drift and fluctuation of sensor signals as well as temperature variation at the same time without time lag. The effectiveness was due to a subtraction function of reference signal from the sample signal. The basic performance of the meter was a drift of within 0.001 degrees in SPR measurement over 4300 s and a angle stability of within 0.0004 degrees at a 10 degrees incident LED light beam. Also, the SPR meter was applied to the immunoassay of 2,4-dichlorophenol (2,4-DCP) using a solid extraction technique. A good linear relationship between the concentration of 2,4-DCP and the SPR signal was obtained with the present meter. It was possible to determine 10 ppb of 2,4-DCP with the developed meter. A new concept of a divided mirror and the Sprode was thought to be useful for miniaturization of the SPR instrument aimed at on-site analysis. (c) 2005 Elsevier B.V. All rights reserved.