Recombinant ferritin protects mice against challenge with Echinococcus granulosus ?
Recombinant ferritin protects mice against challenge with Echinococcus granulosus ?
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DOI：
10.2478/s11686-009-0050-2
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发表时间：
2009-10
影响因子：
1.5
通讯作者：
Yana Wang;Zong-ji Li;Zhaoyu Li;Yang Bo;Wei Zhao
中科院分区：
文献类型：
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作者：
Yana Wang;Zong-ji Li;Zhaoyu Li;Yang Bo;Wei Zhao
众所周知，铁蛋白是原核生物和真核生物中主要的细胞内铁储存蛋白，使其保持可溶且无毒的形式，但铁蛋白作为包虫病候选疫苗的作用尚未明确。通过我们的研究，从细粒棘球绦虫中克隆了铁蛋白，并在大肠杆菌中表达。重组细粒棘球绦虫铁蛋白（rEgferritin）分子量为19 kDa，在Western blotting中可被抗小鼠血清识别。采用酶联免疫吸附法检测特异性抗体。与对照组相比，接种 rEgferritin 并用细粒艾美耳球虫原头节腹腔注射的小鼠显示出显着的保护效果，高达 85.6%。因此，rEgferritin可能成为预防包虫病感染的有效疫苗的有前途的候选者。
Ferritin is well known as the main intracellular iron storage protein in both prokaryotes and eukaryotes, keeping it in a soluble and non-toxic form, though the role of ferritin as a vaccine candidate in echinococcosis has not yet been delineated. Through our study, ferritin was cloned from Echinococcus granulosus and expressed in Escherichia coli. The recombinant E. granulosus ferritin (rEgferritin) has a molecular weight of 19 kDa and could be recognized by anti-mice serum in Western blotting. The specific antibody was detected by enzyme-linked immunosorbent assay. Mice vaccinated with rEgferritin and challenged intraperitoneally with E. granulosus protoscoleces revealed significant protective efficacy up to 85.6%, compared with the control group. Thus, rEgferritin could be a promising candidate as an effective vaccine to prevent the infection of echinococcosis.