Binding Partner Switching on Microtubules and Aurora-B in the Mitosis to Cytokinesis Transition
Binding Partner Switching on Microtubules and Aurora-B in the Mitosis to Cytokinesis Transition
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DOI：
10.1074/mcp.m900308-mcp200
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发表时间：
2010-02-01
影响因子：
7
通讯作者：
Steen, Judith J.
中科院分区：
文献类型：
--
作者：
Ozlu, Nurhan;Monigatti, Flavio;Steen, Judith J.
细胞骨架在有丝分裂(M期)和胞质分裂(C期)之间进行全局重组，这可能需要广泛的调控变化。为了揭示这些变化，我们对每个阶段药物紧密同步的细胞进行了比较蛋白质组学分析。我们鉴定了25个在C期选择性结合到微管的蛋白质，并鉴定了几个新的结合伙伴，包括核仁和纺锤体相关蛋白。其中许多蛋白质的C相选择性微管结合依赖于药物VX680处理所检测到的极光激酶的活性。Aurora-B结合对在M相和C相之间发生了显著的转换，我们鉴定了几个新的C相选择性结合对，包括Prc1、KIf4和后期促进络合物/环体。我们的方法可以扩展到其他细胞间隔和细胞状态，我们的数据为理解C期提供了第一个广泛的生化框架。具体地说，我们报道了Aurora-B在调节C期细胞骨架中的中心作用。《分子与细胞蛋白质组学》9：336-350,2010。
The cytoskeleton globally reorganizes between mitosis (M phase) and cytokinesis (C phase), which presumably requires extensive regulatory changes. To reveal these changes, we undertook a comparative proteomics analysis of cells tightly drug-synchronized in each phase. We identified 25 proteins that bind selectively to microtubules in C phase and identified several novel binding partners including nucleolar and spindle-associated protein. C phase-selective microtubule binding of many of these proteins depended on activity of Aurora kinases as assayed by treatment with the drug VX680. Aurora-B binding partners switched dramatically between M phase to C phase, and we identified several novel C phase-selective Aurora-B binding partners including PRC1, KIF4, and anaphase-promoting complex/cyclosome. Our approach can be extended to other cellular compartments and cell states, and our data provide the first broad biochemical framework for understanding C phase. Concretely, we report a central role for Aurora-B in regulating the C phase cytoskeleton. Molecular & Cellular Proteomics 9:336-350, 2010.