Comparative analysis of microRNA profiles of rice anthers between cool-sensitive and cool-tolerant cultivars under cool-temperature stress.
Comparative analysis of microRNA profiles of rice anthers between cool-sensitive and cool-tolerant cultivars under cool-temperature stress.
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DOI：
10.1266/ggs.15-00056
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发表时间：
2016-03
影响因子：
1.1
通讯作者：
S. Maeda;Satomi Sakazono;Hiromi Masuko-Suzuki;Midori Taguchi;Kaori Yamamura;K. Nagano;T. Endo;
中科院分区：
文献类型：
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作者：
S. Maeda;Satomi Sakazono;Hiromi Masuko-Suzuki;Midori Taguchi;Kaori Yamamura;K. Nagano;T. Endo;
受到非生物胁迫的植物可以通过小RNA如microRNA在转录后调节基因表达。低温胁迫导致水稻花药绒毡层异常肥大，导致花粉不育。作为理解水稻花药发育中耐冷性分子机制的第一步，我们在这里报告了冷敏感的Sasanishiki和耐冷的Hitomebore品种之间的microRNA的全面比较分析。高通量Illumina测序揭示了241种已知的和46种新的microRNA。有趣的是，这些microRNA中的15个在两个品种的单核小孢子阶段在低温条件下差异积累。通过定量RT-PCR分析证实了microRNA的表达模式与其靶基因之间的负相关性，并通过5' RNA连接酶介导的RACE实验确定了一些靶基因的切割位点。因此，我们的数据是有用的资源，以阐明microRNA介导的耐冷性在水稻花药孕穗期的机制（S）。
Plants subjected to abiotic stress can regulate gene expression post-transcriptionally by means of small RNAs such as microRNAs. Cool-temperature stress causes abnormal tapetum hypertrophy in rice anthers, leading to pollen sterility. As a first step toward understanding the molecular mechanisms of cool tolerance in developing anthers of rice, we report here a comprehensive comparative analysis of microRNAs between cool-sensitive Sasanishiki and cool-tolerant Hitomebore cultivars. High-throughput Illumina sequencing revealed 241 known and 46 novel microRNAs. Interestingly, 15 of these microRNAs accumulated differentially in the two cultivars at the uninucleate microspore stage under cool conditions. Inverse correlations between expression patterns of microRNAs and their target genes were confirmed by quantitative RT-PCR analysis, and cleavage sites of some of the target genes were determined by 5' RNA ligase-mediated RACE experiments. Thus, our data are useful resources to elucidate microRNA-mediated mechanism(s) of cool tolerance in rice anthers at the booting stage.