Effects on human skin of repetitive ultraviolet-A1 (UVA1) irradiation and visible light
Effects on human skin of repetitive ultraviolet-A1 (UVA1) irradiation and visible light
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DOI：
10.1034/j.1600-0781.2001.017002066.x
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发表时间：
2001-04-01
影响因子：
2.6
通讯作者：
Ros, AM
中科院分区：
文献类型：
--
作者：
Edström, DW;Porwit, A;Ros, AM
背景：紫外线辐射（UVR）对人体皮肤有多种影响。最著名的是UVB（290-320 nm）和UVA 2（320-340 nm）的作用，其引起DIVA损伤和癌症风险增加，然而，UVA 1（340-400 nm）的作用尚未完全研究。一部分皮肤暴露于20 J/cm（2）的UVA 1，另一部分暴露于126 J/cm（2）的可见光，每周3次，持续4周。重复穿刺活检：在治疗4周期间以及末次照射后2周进行。结果：与对照组相比，茶组角质形成细胞中p53蛋白表达增加，而p21蛋白表达无明显变化。结论：低剂量的UVA 1甚至可见光照射均可引起细胞DNA的损伤。
Background: Ultraviolet radiation (UVR) has a variety of effects on human skin. Best known are the effects of UVB (290-320 nm) and UVA2 (320-340 nm), which cause DIVA damage and increased risk of cancer, However, the effects of UVA1 (340-400 nm) have been not completely investigated.Methods: The effects of repetitive low doses of UVA1 and visible light were studied in 12 healthy individuals. A part of the buttock was exposed to 20 J/cm(2) UVA1 and another part to 126 J/cm(2) of visible light three times a week for 4 weeks. Repeated punch biopsies: were taken during the 4 weeks of treatment and also 2 weeks after the last irradiation. The avidin-biotin-immunoperoxidase technique was used to investigate the expression of p53, p21(WAF), bcl-2, Ki67 and cyclin A.Results: By comparison tea untreated skin, an increased expression of p53 but not p21(WAF) in keratinocytes was seen. The bcl-2 protein expression increased slightly after both UVA1 and visible light, An increased staining with Ki67 and cyclin A after UVA1 but not after visible light was observed as a sign of increased proliferation.Conclusion: These results suggest that suberythemal doses of UVA1 and even visible light: may cause DNA damage.