Characterization of antioxidant properties of natural killer-enhancing factor-B and induction of its expression by hydrogen peroxide.
Characterization of antioxidant properties of natural killer-enhancing factor-B and induction of its expression by hydrogen peroxide.
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自然杀伤增强因子 B 抗氧化特性的表征及其过氧化氢诱导表达。
DOI：
10.1006/taap.1997.8270
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发表时间：
1997
期刊：
影响因子：
--
通讯作者：
Shau,H
中科院分区：
文献类型：
--
作者：
Kim,AT;Sarafian,TA;Shau,H
自然杀伤增强因子B(NKEF-B)属于新近发现的高度保守的抗氧化剂家族。NKEF-B对过氧化氢氧化损伤的保护作用证明了其作为抗氧化剂的作用。为了进一步研究NKEF-B的抗氧化特性，我们比较了人内皮细胞系ECV304及其高表达NKEF-B的转染体B/1对不同氧化剂的敏感性。此外，我们还研究了氧化应激对NKEF-B基因表达的影响。我们发现B/1细胞对叔丁基氢过氧化氢(t-BHP)和甲基汞(MeHg)引起的氧化应激的抵抗力明显高于对照细胞。B/1细胞和对照细胞对巯基反应物、顺丁烯二酸二乙酯和联胺的敏感性无差异。B/1细胞对丁硫氨酸亚磺胺的敏感性与对照细胞相同。2 mMHP可诱导亲本细胞株ECV304表达NKEF-B。诱导在2小时左右达到最大水平，在4小时左右下降到基础水平。幽门螺杆菌不能诱导NKEF-A基因的表达。这些结果表明，NKEF-B对烷基过氧化氢和甲基汞等促氧化剂具有抗氧化活性。幽门螺杆菌对NKEF-B的诱导结合其抗氧化活性，表明NKEF-B是一种重要的氧化应激蛋白，对多种外来毒物具有保护作用。
Natural killer-enhancing factor B (NKEF-B) belongs to a highly conserved family of recently discovered antioxidants. The role of NKEF-B as an antioxidant was demonstrated by its protection of transfected cells to oxidative damage by hydrogen peroxide. To further characterize the antioxidant properties of NKEF-B, we compared the sensitivity of a human endothelial cell line ECV304 and its transfectant, B/1 that hyperexpresses NKEF-B, to various oxidants. In addition, we investigated the changes in the expression of NKEF-B mRNA upon oxidative stress. We found that B/1 was significantly more resistant than the control cells to the oxidative stresses caused byt-butyl hydroperoxide (t-BHP) and methyl mercury (MeHg). In contrast, there was no difference in the sensitivity of B/1 and the control cells to sulfhydryl reactive agents, diethyl maleate and diamide. B/1 was also as sensitive as the control cells to buthionine sulfoximine. The expression of NKEF-B mRNA was induced when the parental cell line ECV304 was treated with 2 mmHP. The induction reached a maximum level around 2 hr and decreased to the basal level around 4 hr. NKEF-A mRNA was not induced by HP. These results demonstrate antioxidant activities of NKEF-B toward prooxidants such as alkyl hydroperoxide and MeHg. Together with its antioxidant activity, the induction of NKEF-B by HP indicates that NKEF-B is an important oxidative stress protein providing protection against a variety of xenobiotic toxic agents.
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DOI：
10.1073/pnas.91.15.7017
发表时间：
1994-07-19
影响因子：
11.1
作者：
CHAE, HZ;ROBISON, K;RHEE, SG
通讯作者：
RHEE, SG
影响因子：
7.4
作者：
H. Shau;A. Kim;C. Hedrick;A. J. Lusis;C. Tompkins;R. Finney;D. Leung;D. Paglia
通讯作者：
H. Shau;A. Kim;C. Hedrick;A. J. Lusis;C. Tompkins;R. Finney;D. Leung;D. Paglia
影响因子：
4.3
作者：
SHAU, HY;GUPTA, RK;GOLUB, SH
通讯作者：
GOLUB, SH
DOI：
10.1073/pnas.87.16.6358
发表时间：
1990-08-01
影响因子：
11.1
作者：
TORIAN, BE;FLORES, BM;STAMM, WE
通讯作者：
STAMM, WE
人心脏肌动蛋白基因启动子中的多个 CArG 盒是在胚胎癌细胞体外发育的胚胎心肌细胞中表达所需的
影响因子：
5.3
作者：
G. Pari;K. Jardine;M. McBurney
通讯作者：
M. McBurney