Localization of protein A on the cell surface of Staphylococcus aureus Cowan I and protein A-deficient strains.
Localization of protein A on the cell surface of Staphylococcus aureus Cowan I and protein A-deficient strains.
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金黄色葡萄球菌 Cowan I 和蛋白 A 缺陷菌株细胞表面蛋白 A 的定位。
DOI：
10.1093/oxfordjournals.jmicro.a050452
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发表时间：
1984
期刊：
影响因子：
--
通讯作者：
A. Matsumoto
中科院分区：
文献类型：
--
作者：
S. Yamada;A. Matsumoto
金黄色葡萄球菌(S.aureus)具有一种表面成分--蛋白A，它具有与大多数哺乳动物血清中免疫球蛋白G分子的Fc部分发生反应的独特性质。1“针对这一性质，我们研究了Cowan I和Wood 46菌株的细胞表面蛋白A的定位，并保留了由Saitama医学院的Y.Arai博士亲手提供的前者的缺乏金黄色葡萄球菌蛋白的HL-87，4‘，Cowan I和Wood 46菌株。通过对Masuda et a/方法的改进，从Cowan I菌株中分离到5株A蛋白缺陷株。61对其中一株菌株HL-87与Cowan I和Wood 46菌株进行了联合检测。用溶葡萄球菌酶(5个单位/毫升，美国西格玛化学公司生产)处理约10“菌落单位/毫升的细菌细胞，以2,300g的速度离心除去细胞碎片，上清液用Spika等人描述的血凝试验进行检测。
Staphylococcus aureus (S. aureus) possess a surface component, protein A, which has the unique property of reacting with the Fc portion of the IgG molecule of most mammalian sera. 1"" Specifically with regard to this property, we studied the localization of protein A on the cell surface of the Cowan I and Wood 46 strains, and HL-87, 4'an isolate of the former which lacks protein A. S. aureus, Cowan I and Wood 46 strains, which were kindly supplied by Dr. Y. Arai, Saitama Medical School, were maintained in our laboratory. Five protein A-deficient strains were isolated from the Cowan I strain by a modification of the method of Masuda et a/. 61 One of the isolates, HL-87, was examined together with the Cowan I and Wood 46 strains. The bacterial cells of about 10" colonyforming units/ml were treated with lysostaphin (5 units/ml, Sigma Chemical Co., USA) and centrifuged at 2,300 g to remove the cell debris. The supernatant was assayed by the hemagglutination test described by Spika et al. e) The titer was expressed as the reciprocal of the highest dilution showing visible hemagglutination.