OsSEND-1:: a new RAD2 nuclease family member in higher plants
OsSEND-1:: a new RAD2 nuclease family member in higher plants
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DOI：
10.1023/a:1020789314722
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发表时间：
2003-01-01
影响因子：
5.1
通讯作者：
Sakaguchi, K
中科院分区：
文献类型：
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作者：
Furukawa, T;Kimura, S;Sakaguchi, K
从高等植物水稻（Oryza sativa L.）CV. Nipponbare），并命名为OsSEND-1。OsSEND-1 cDNA的开放阅读框编码641个氨基酸残基，分子量为69.9 kDa。编码的蛋白质显示出与RAD 2核酸酶家族蛋白，尤其是RAD 2核酸酶的相对高度的序列同源性，但它与FEN-1、XPG或HEX 1/EXO 1显著不同。OsSEND-1基因的N-和I-结构域高度保守。该蛋白比XPG小得多，但比HEX 1/EXO 1和FEN-1大。基因组序列由14个外显子组成，定位于8号染色体短臂的近末端区域。北方印迹和原位杂交分析表明OsSEND-1 mRNA在分生组织等增殖组织中优先表达。OsSEND-1的mRNA水平在紫外线和DNA损伤剂如MMS或H2 O2的诱导下表达，表明OsSEND-1在多种类型的DNA损伤的修复中具有一定的作用。重组肽具有核酸内切酶活性。
A novel endonuclease, a new member of the RAD2 nuclease family, has been identified from the higher plant, rice (Oryza sativa L. cv. Nipponbare), and designated as OsSEND-1. The open reading frame of the OsSEND-1 cDNA encoded a predicted product of 641 amino acid residues with a molecular weight of 69.9 kDa. The encoded protein showed a relatively high degree of sequence homology with the RAD2 nuclease family proteins, especially RAD2 nuclease, but it differed markedly from FEN-1, XPG or HEX1/EXO1. The N- and I-domains in the family were highly conserved in the OsSEND-1 sequence. The protein was much smaller than XPG, but larger than HEX1/EXO1 and FEN-1. The genome sequence was composed of 14 exons, and was localized at the almost terminal region of the short arm of chromosome 8. Northern blotting and in situ hybridization analyses demonstrated preferential expression of OsSEND-1 mRNA in proliferating tissues such as meristem. The mRNA level of OsSEND-1 was induced by UV and DNA-damaging agent such as MMSor H2O2, indicating that OsSEND-1 has some roles in the repair of many types of damaged DNA. The recombinant peptide showed endonuclease activity.